目的 觀察人牙髓間充質(zhì)干細(xì)胞（HDP-MSCs）對免疫缺陷小鼠的致瘤性作用。方法 雌性NOG小鼠隨機(jī)分為4組：對照組和HDP-MSCs低、中、高劑量（2×10⁶、5×10⁶和2×10⁷ cells/kg）組，每組20只；雙后肢交替im給藥，每周給藥1次，共給藥6個(gè)月（27次）；試驗(yàn)期間進(jìn)行大體觀察、體質(zhì)量檢查和腫瘤觸診，給藥結(jié)束進(jìn)行解剖檢查、臟器檢查和組織病理學(xué)檢查。結(jié)果 對照組死亡2只動物，其中1只動物頸部出現(xiàn)腫塊；HDP-MSCs低劑量死亡1只動物，另有1只動物出現(xiàn)弓背；中劑量組1只動物瀕死；高劑量動物未見明顯異常；低、中、高劑量組觸診未發(fā)現(xiàn)腫瘤。小鼠體質(zhì)量無異常。解剖后對照組和HDP-MSCs低、中劑量個(gè)別動物發(fā)現(xiàn)有腫塊。各劑量組臟器質(zhì)量及系數(shù)無異常、組織病理學(xué)檢查無藥物相關(guān)性病變。結(jié)論 HDP-MSCs在本試驗(yàn)劑量下連續(xù)im給藥6個(gè)月，致瘤試驗(yàn)結(jié)果為陰性。

Objective To evaluate tumorigenicity of human dental pulp mesenchymal stem cells (HDP-MSCs) on NOG mice. Methods NOG mice were divided into four groups (20 mice each group), which included low, medium, high dose (2×10⁶、5×10⁶ and 2×10⁷ cells/kg) groups and one blank control group. The mice were given HDP-MSCs by alternate intramuscular injection in both hind limbs once a week and this operation last for 6 months (27 times in total). Clinical observation, weight examination and tumor palpation on NOG mice were conducted in experimental observation period. Gross anatomy, organs and histopathology on NOG mice were examined at the end of the experiment.Result Two animals of the blank control group died, one of which had a neck mass. One animal died in the low dose group, and another animal observed one symptoms of arched back. One animal in the middle dose group was in a state of near death. The animals in the high dose group were no obvious abnormality. There was no tumor found on tumor palpation in the low, medium and high dose groups. The mice weight was normal. Tumor was found on autopsy in blank control group, low and medium dose group. Viscera weight and coefficient was no abnormality. No drug-related lesion was found in histopathology.Conclusion The result of tumorigenicity test of HDP-MSCs on NOG mice was negative.

R329

天津市科技計(jì)劃項(xiàng)目（17ZXXYSY00020）