目的 探索人臍帶間充質(zhì)干細(xì)胞復(fù)方電解質(zhì)注射液制劑穩(wěn)定性。方法 人臍帶間充質(zhì)干細(xì)胞（密度5×10⁵/mL）分別與復(fù)方電解質(zhì)注射液及0.9%氯化鈉注射液（分別設(shè)加/不加2%人血白蛋白組）制備制劑，通過觀察24 h內(nèi)不同時(shí)間節(jié)點(diǎn)凋亡變化趨勢(shì)優(yōu)選出制劑方案；探討該制劑的光照穩(wěn)定性、熱循環(huán)穩(wěn)定性、加速穩(wěn)定性以及長(zhǎng)期穩(wěn)定性。結(jié)果 復(fù)方電解質(zhì)注射液制劑較0.9%氯化鈉注射液制劑細(xì)胞凋亡水平低；2%白蛋白添加能夠有效降低細(xì)胞凋亡水平。光照對(duì)復(fù)方電解質(zhì)注射液添加2%白蛋白細(xì)胞制劑無嚴(yán)重影響；溫度波動(dòng)能導(dǎo)致細(xì)胞存活率快速下降；高溫對(duì)細(xì)胞制劑狀態(tài)有較大影響，導(dǎo)致絮狀物大量出現(xiàn)，細(xì)胞存活率快速下降；在低溫條件下，該制劑能長(zhǎng)時(shí)間保持細(xì)胞存活率。結(jié)論 復(fù)方電解質(zhì)注射液添加2%白蛋白是一種優(yōu)良的人臍帶間充質(zhì)干細(xì)胞制劑方法。

Objective To explore a preparation method of human umbilical cord mesenchymal stem cells and its stability in Multiple Electrolytes Injection. Methods Observed the apoptosis levels of human umbilical cord mesenchymal stem cells (5×10⁵/mL) in Sodium Chloride Injection and Multiple Electrolytes Injection (2% human serum albumin was added or not). The best preparation was further studied, which included light stability, thermal cycle stability, accelerated stability and long-term stability. Results The apoptosis level of Multiple Electrolytes Injection preparation was lower than that of 0.9% sodium chloride injection, and 2% albumin could effectively reduce the apoptosis level. The results showed that light had no serious effect on the cell preparation supplemented with 2% albumin; the fluctuation of temperature could lead to the rapid decrease of cell survival rate; the high temperature had a great influence on the state of cell preparation, which led to the rapid decline of cell survival rate; under the condition of low temperature, the preparation could maintain the cell survival rate for a long time. Conclusion Multiple Electrolytes Injection is a good preparation method for stem cell.

R329

國(guó)家重點(diǎn)研發(fā)計(jì)劃“干細(xì)胞及轉(zhuǎn)化研究”重點(diǎn)專項(xiàng)（2019YFA0112100）；天津市科技計(jì)劃項(xiàng)目（17ZXXYSY00020）