目的 研究小兒治哮靈片對幼年大鼠哮喘模型的干預(yù)作用及機(jī)制。方法 36只雄性Wistar幼年大鼠隨機(jī)分為6組：對照組、模型組、醋酸地塞米松（陽性藥，1 mg/kg）組和小兒治哮靈片低、中、高劑量（0.32、0.64、1.28 g/kg）組。除對照組外，其余各組以卵清蛋白（OVA）致敏、激發(fā)制備幼年大鼠哮喘模型。造模第8天開始ig給藥，每天給藥1次，給藥至造模3周，對照組和模型組ig給予蒸餾水。記錄各組大鼠引喘潛伏期并進(jìn)行行為學(xué)評分；檢測肺臟器指數(shù)；酶聯(lián)免疫吸附法（ELISA）檢測各組幼年大鼠血清中白細(xì)胞介素（IL）-4、IL-5、IL-6、免疫球蛋白E（IgE）、γ-干擾素（IFN-γ）、核轉(zhuǎn)錄因子（NF-κB）的水平以及肺泡灌洗液中IFN-γ水平。結(jié)果 與模型組比較，小兒治哮靈片高、中、低劑量組引喘潛伏期及哮喘行為評分均顯著下降（P<0.05、0.01）；肺臟器指數(shù)顯著下降（P<0.05）；血清IL-4、IL-5、IL-6、IgE、NF-κB水平均顯著下降（P<0.01）；血清與肺泡灌洗液中IFN-γ水平顯著升高（P<0.01）。結(jié)論 小兒治哮靈片抗哮喘作用可能與降低炎性細(xì)胞因子分泌有關(guān)。

Objective To study the interventional effect and mechanism of Xiaoer Zhixiaoling Tablets on asthmatic model in juvenile rats. Methods Thirty six young male Wistar rats were randomly divided into six groups:control group, model group, dexamethasone acetate (positive drug, 1 mg/kg) group and low, medium and high dose (0.32, 0.64, 1.28 g/kg) groups of Xiaoerzhixiaoling Tablets. Expect the blank control group, the other groups of young rats were sensitized by egg albumin (OVA) and stimulated to prepare the rat model of asthma in juvenile rats. On the 8th day of modeling, drugs were ig given once a day until 3 weeks for modeling. The control group and model group were given distilled water. The incubation period of asthma was recorded and the behavior score was evaluated. The levels of IL-4、IL-5、IL-6、IgE、IFN-γ、NF-κB in serum of young rats and the level of IFN-γ in alveolar lavage were detected by enzyme-linked immunosorbent method. Results Compared with the model group, the asthma latency and asthma behavior scores in the high, middle and low dose groups of Xiaoer Zhixiaoling Tablets were significantly decreased (P<0.05 and 0.01), and the lung organ index was significantly decreased (P<0.05); levels of IL-4, IL-5, IL-6, IgE, NF-κB decreased in the highdose group, the medium-dose group and the low-dose group (P<0.01), and levels of IFN-γ in serum and alveolar lavage fluid increased (P<0.01). Conclusion The anti-asthma effect of Xiaoer Zhixiaoling Tablets; may be related to the decreased secretion of downstream inflammatory cytokines.

R285.5

校企合作項(xiàng)目“小兒治哮靈片安全性評價(jià)研究”資助