目的 探究甘草次酸對(duì)胃癌SGC7901細(xì)胞增殖、凋亡及PI3K-Akt-mTOR通路的影響。方法 體外培養(yǎng)胃癌SGC7901細(xì)胞，分為對(duì)照組和甘草次酸低、中、高濃度（12.5、25.0、50.0 μmol/L）組，藥物處理24、48、72 h后，CCK-8法檢測(cè)細(xì)胞增殖情況；藥物處理48 h后，流式細(xì)胞儀檢測(cè)細(xì)胞周期分布及細(xì)胞凋亡情況；Western blotting檢測(cè)凋亡相關(guān)蛋白Bcl-2、Bax、cleaved Caspase-3以及PI3K-Akt-mTOR信號(hào)通路中磷酸化PI3K、Akt、mTOR蛋白表達(dá)水平。結(jié)果 甘草次酸低、中、高濃度組胃癌SGC7901細(xì)胞增殖受到明顯抑制，并呈時(shí)間和劑量相關(guān)性（P<0.05）。與對(duì)照組比較，甘草次酸低、中、高濃度組胃癌SGC7901細(xì)胞G₀/G₁期細(xì)胞比例顯著降低（P<0.05），G₂/M期細(xì)胞比例及凋亡率顯著升高（P<0.05），Bax、cleaved Caspase-3蛋白表達(dá)水平顯著升高（P<0.05），Bcl-2、p-PI3K、p-Akt、p-mTOR蛋白表達(dá)水平顯著降低（P<0.05）。結(jié)論 甘草次酸可抑制胃癌SGC7901細(xì)胞增殖，促進(jìn)其凋亡，其機(jī)制可能與抑制PI3K-Akt-mTOR信號(hào)通路激活有關(guān)。

Objective To investigate the effects of glycyrrhetinic acid on proliferation, apoptosis and phosphatidylinositol 3-kinase (PI3K) -serine/threonine protein kinase (Akt) -mammalian target of rapamycin (mTOR) (PI3K-Akt-mTOR) pathway of gastric cancer SGC7901 cells. Methods Gastric cancer SGC7901 cells were cultured in vitro, and divided into control group, low, medium and high concentration (12.5, 25.0 and 50.0 μmol/L) groups of glycyrrhetinic acid. Cells proliferation was detected by CCK-8; Cells cycle distribution and apoptosis were detected by flow cytometry; The apoptosis-associated proteins Bcl-2, Bax, cleaved Caspase-3, and phosphorylation of PI3K, Akt and mTOR protein PI3K-Akt-mTOR signaling pathway expression levels were analysed by Western blotting. Results The proliferation of gastric cancer SGC7901 cells in low, medium and high concentrations groups of glycyrrhetinic acid was significantly inhibited in a time- and dose-dependent manner (P<0.05). Compared with the control group, in the low, middle and high concentration groups of glycyrrhetinic acid, the proportion of G₀/G₁ phase cells of gastric cancer SGC7901 cells significantly decreased (P<0.05), and the proportion of G₂/M phase cells and the apoptosis rate significantly increased (P<0.05). The expression levels of Bax and cleaved Caspase-3 proteins significantly increased (P<0.05), and the expression levels of Bcl-2, p-PI3K, p-Akt and p-mTOR proteins significantly decreased (P<0.05).Conclusion Glycyrrhetinic Acid can inhibit the proliferation and promote the apoptosis of gastric cancer SGC7901 cells, which may be related to the inhibition of PI3K-Akt-mTOR signaling pathway.

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