0/G1期細(xì)胞比例顯著降低(P<0.05),G2/M期細(xì)胞比例及凋亡率顯著升高(P<0.05),Bax、cleaved Caspase-3蛋白表達(dá)水平顯著升高(P<0.05),Bcl-2、p-PI3K、p-Akt、p-mTOR蛋白表達(dá)水平顯著降低(P<0.05)。結(jié)論 甘草次酸可抑制胃癌SGC7901細(xì)胞增殖,促進(jìn)其凋亡,其機(jī)制可能與抑制PI3K-Akt-mTOR信號(hào)通路激活有關(guān)。;Objective To investigate the effects of glycyrrhetinic acid on proliferation, apoptosis and phosphatidylinositol 3-kinase (PI3K) -serine/threonine protein kinase (Akt) -mammalian target of rapamycin (mTOR) (PI3K-Akt-mTOR) pathway of gastric cancer SGC7901 cells. Methods Gastric cancer SGC7901 cells were cultured in vitro, and divided into control group, low, medium and high concentration (12.5, 25.0 and 50.0 μmol/L) groups of glycyrrhetinic acid. Cells proliferation was detected by CCK-8; Cells cycle distribution and apoptosis were detected by flow cytometry; The apoptosis-associated proteins Bcl-2, Bax, cleaved Caspase-3, and phosphorylation of PI3K, Akt and mTOR protein PI3K-Akt-mTOR signaling pathway expression levels were analysed by Western blotting. Results The proliferation of gastric cancer SGC7901 cells in low, medium and high concentrations groups of glycyrrhetinic acid was significantly inhibited in a time- and dose-dependent manner (P<0.05). Compared with the control group, in the low, middle and high concentration groups of glycyrrhetinic acid, the proportion of G0/G1 phase cells of gastric cancer SGC7901 cells significantly decreased (P<0.05), and the proportion of G2/M phase cells and the apoptosis rate significantly increased (P<0.05). The expression levels of Bax and cleaved Caspase-3 proteins significantly increased (P<0.05), and the expression levels of Bcl-2, p-PI3K, p-Akt and p-mTOR proteins significantly decreased (P<0.05).Conclusion Glycyrrhetinic Acid can inhibit the proliferation and promote the apoptosis of gastric cancer SGC7901 cells, which may be related to the inhibition of PI3K-Akt-mTOR signaling pathway."/>

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首頁(yè) > 過(guò)刊瀏覽>2020年第43卷第12期 >2020,43(12):2444-2449. DOI:10.7501/j.issn.1674-6376.2020.12.016
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甘草次酸對(duì)胃癌細(xì)胞SGC7901增殖、凋亡及PI3K-Akt-mTOR通路的影響

Effects of glycyrrhetinic acid on proliferation, apoptosis, and PI3K-AKT-mTOR pathway of gastric cancer cells SGC7901

發(fā)布日期:2020-12-04
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