Ca,L電流密度顯著減弱,差異有統(tǒng)計學(xué)意義(P<0.05、0.01)。與對照組比較,BayK 8644組ICa,L的電流-電壓(I-V)曲線顯著下移,電流密度顯著增強(P<0.01),使半數(shù)激活電壓明顯升高3.23倍,激活曲線左移,激活加快。TMCC (0.01、0.1、1 mol/L)可明顯減弱BayK 8644對ICa,L電流的增強作用,使下移的I-V曲線上移,0.1、1 mol/L濃度組差異有統(tǒng)計學(xué)意義(P<0.05、0.01);TMCC各濃度組均明顯降低半數(shù)激活電壓(P<0.05、0.01),恢復(fù)左移的激活曲線,使激活減慢。在豚鼠心室肌細(xì)胞中,與對照組比較,BayK 8644顯著延長30%、50%和90%復(fù)極化動作電位持續(xù)時間(APD30、APD50和APD90)(P<0.01);0.01、0.1、1 mmol/L TMCC均可以減弱BayK 8644對APD30、APD50和APD90的延長作用,0.1、1 mmol/L濃度組差異顯著(P<0.05、0.01)。結(jié)論 TMCC通過縮短動作電位時程,減弱被增強的ICa,L電流,發(fā)揮一定的抗LQT8的作用。;Objective In this study, the anti-arrhythmic effect of Taurine-Magnesium Coordination Compound (TMCC) was assessed on the model of type 8 long QT syndrome (LQT8). Methods BayK 8644 (10 nmol/L) was used to establish the LQT8 model in guinea pig ventricular myocytes and to enhance HEK293 cells stably expressing ICa,L channels. All cells were incubated for 24 h in the absence and presence of drugs. The ICa, L current and action potentials were recorded by using the whole-cell patch-clamp technique. Results In HEK293 cells, compared with control group, the ICa, L current density of TMCC 0.1 and 1 mol/L groups was significantly decreased (P<0.05 and 0.01). Compared with control group, the current voltage (I-V) curve of ICa, L in BayK 8644 group decreased significantly, the current density increased significantly (P<0.01), the half activation voltage increased 3.23 times, the activation curve shifted to the left, and the activation accelerated. TMCC (0.01, 0.1, 1 mol/L) significantly reduced the enhancement of ICa, L current by BayK 8644, and made the downward I-V curve move up, with significant difference between 0.1, 1 mol/L concentration groups (P<0.05 and 0.01). All TMCC concentration groups significantly reduced the half activation voltage (P<0.05 and 0.01), restored the left activation curve, and slowed down the activation. In guinea pig ventricular myocytes, BayK 8644 significantly prolonged the duration of repolarization action potential (APD30, APD50 and APD90) compared with control group (P<0.01). 0.01, 0.1 and 1 mmol/L TMCC could attenuate the prolongation of APD30, APD50 and APD90 by BayK 8644, with significant differences between 0.1 and 1 mmol/L TMCC groups (P<0.05 and 0.01). Conclusion It was suggested that TMCC have anti-arrhythmic effect on LQT8 through shortening the action potential duration and inhibiting the increased ICa, L current."/>