目的 基于序貫代謝的研究思路，研究白芍Paeoniae Radix Alba多種成分在體內(nèi)的代謝情況，遴選白芍潛在質(zhì)控成分。方法 制備白芍水提物，通過在體動(dòng)物實(shí)驗(yàn)的方法，收集白芍的腸代謝、肝代謝以及綜合代謝樣品，結(jié)合超高效液相色譜-四級(jí)桿/靜電場(chǎng)高分辨質(zhì)譜聯(lián)用技術(shù)（UPLC-Q-Exacitve MS），對(duì)各個(gè)樣品所含成分進(jìn)行分析與鑒別。結(jié)果 白芍水提物中共鑒定出36個(gè)化學(xué)成分。序貫代謝結(jié)果顯示，共有17個(gè)原型入血成分，其中有14個(gè)成分在經(jīng)腸代謝樣品中檢測(cè)到，有8個(gè)成分先經(jīng)腸代謝、再經(jīng)肝代謝后可檢測(cè)到，有11個(gè)成分在經(jīng)ig給藥的綜合代謝樣品中檢測(cè)到；羥基芍藥苷、芍藥苷、芍藥內(nèi)酯苷、環(huán)五（異）亮氨酸、環(huán)六（異）亮氨酸、苯甲酸、對(duì)羥基苯甲酸這7個(gè)成分在3種代謝部位均可以檢測(cè)到。結(jié)論 共遴選出沒食子酸、芍藥內(nèi)酯苷等17個(gè)經(jīng)代謝后仍原型入血的成分，可作為日后白芍質(zhì)控成分。

Objective To investigate the metabolism of Radix Paeoniae Alba and to select potential quality control components based on the research through of sequential metabolism. Methods After preparing the water extract of Radix Paeoniae Alba, in vivo animal experiments were applied to collect its intestinal metabolism, liver metabolism, as well as comprehensive metabolism samples, combined with ultra-high performance liquid chromatography-quadrupole/electrostatic field high-resolution mass spectrometry(UPLC-Q-Exactive MS), to analyze and identify the components of samples. Results A total of 36 chemical components were identified from the water extract of Radix Paeoniae Alba. Sequential metabolic studies shown that there were 17 protoplasmic components, of which 14 components could be detected after intestinal metabolism, 8 components could be detected after intestinal metabolism followed by liver metabolism, and 11 components could be detected in comprehensive metabolic samples after intragastric administration. Hydroxypaeoniflorin, paeoniflorin, paeoniflorin, cyclopenta (isoleucine), cyclohexa (isoleucine), benzoic acid and p-hydroxybenzoic acid were detected in the three metabolic sites. Conclusion Through analyzing the results of different samples, 17 components that still can enter the blood circulation in the form of a prototype after metabolism, such as gallic acid, albiflorin and so on, were selected as potential quality control components of Radix Paeoniae Alba in the future.

R962.2

國(guó)家中醫(yī)藥管理局項(xiàng)目（ZYBZH-Y-HUB-20）