目的 建立SD大鼠代謝相關(guān)脂肪性肝病（metabolic associated fatty liver disease，MAFLD）模型，觀察大鼠病理變化。方法 用高糖高脂飼料飼喂雄性SD大鼠20周，檢測(cè)模型組大鼠血清和肝勻漿相關(guān)生化指標(biāo)的水平、肝臟臟器質(zhì)量和系數(shù)、肝臟組織病理學(xué)變化。結(jié)果 模型組大鼠飼喂高糖高脂飼料20周后，與對(duì)照組比較，血清總膽固醇（TC）、低密度脂蛋白膽固醇（LDL-C）及肝臟TC和三酰甘油（TG）的水平均顯著升高（P<0.01），血清TG、高密度脂蛋白膽固醇（HDLC）的水平均顯著降低（P<0.01），肝臟質(zhì)量及系數(shù)顯著性增加（P<0.01）。肝組織的病理改變?yōu)閲?yán)重肝細(xì)胞脂肪變性及多灶性肉芽腫性炎。結(jié)論 高糖高脂飼料飼喂成功建立了合適的MAFLD動(dòng)物模型，肝臟病理改變可見(jiàn)廣泛的脂肪變性和大量的肉芽腫性炎癥，未見(jiàn)纖維化。

Objectives To establish a model of metabolic associated fatty liver disease (MAFLD) in Sprague-Dawley rats. Methods 30 5-week-old male Sprague-Dawley rats in the model group were fed with high-sucrose-fat diet for 20 weeks, another 30 rats in the control group were fed with normal diet. The following indicators were evaluated:3 biochemical indexes in the serum and liver homogenate, organ weight and ratio, histopathological changes of the liver. Results Compared with the control group, the rats in model group showed significant increase in the level of total cholesterol (TC), triglyceride (TG), low density lipoprotein chesterol (LDL-C) and high density lipoprotein chesterol (HDL-C) in serum or liver homogenate. Also, the organ weight and ratio of liver in model group were increased significantly. Moreover, severe hepatocyte steatosis and multifocal granulomatous inflammation were observed in the livers of model group. Conclusions An appropriate rat model of MAFLD was successfully established by fed with high-sucrose-fat diet for 20 weeks. Severe hepatocyte steatosis and multifocal granulomatous inflammation were observed in the livers of model group and no fibrosis were found.

R965.1

國(guó)家科技重大專項(xiàng)重大新藥創(chuàng)制（2018ZX09721004-001-003）