目的探討刺槐素對(duì)肺癌細(xì)胞增殖、遷移和侵襲的影響及分子機(jī)制。方法用濃度分別為4、8、16 μmol/L的刺槐素處理A549細(xì)胞，作為低、中、高濃度組，不作任何處理的細(xì)胞作為對(duì)照組；將pcDNA、pcDNA-LZTFL1轉(zhuǎn)染至A549細(xì)胞中，記為pcDNA組、pcDNA-LZTFL1組；將si-NC、si-LZTFL1轉(zhuǎn)染至A549細(xì)胞中再用16 μmol/L的刺槐素處理，記為刺槐素+si-NC組、刺槐素+si-LZTFL1組。四甲基偶氮唑鹽比色法（MTT）檢測(cè)細(xì)胞增殖抑制率；Western blotting法檢測(cè)LZTFL1、CyclinD1、p21、MMP-2、MMP-9蛋白表達(dá)；Transwell檢測(cè)細(xì)胞遷移和侵襲；實(shí)時(shí)熒光定量PCR（qRT-PCR）檢測(cè)LZTFL1 mRNA表達(dá)水平。結(jié)果與對(duì)照組比較，刺槐素低、中、高濃度處理的肺癌A549細(xì)胞中細(xì)胞增殖抑制率顯著升高，細(xì)胞遷移、侵襲數(shù)顯著降低，CyclinD1、MMP-2、MMP-9蛋白表達(dá)水平顯著降低，p21蛋白表達(dá)水平顯著升高，LZTFL1 mRNA和蛋白表達(dá)水平顯著升高（P<0.05）。過(guò)表達(dá)LZTFL1抑制肺癌細(xì)胞的增殖、遷移和侵襲。干擾LZTFL1表達(dá)逆轉(zhuǎn)了刺槐素對(duì)肺癌A549細(xì)胞增殖、遷移和侵襲的抑制作用。結(jié)論刺槐素可能通過(guò)上調(diào)LZTFL1的表達(dá)抑制肺癌細(xì)胞的增殖、遷移和侵襲。

Objective To investigate the effect of acacetin on the proliferation, migration and invasion of lung cancer cells and its molecular mechanism. Methods A549 cells were treated with acacetins at concentrations of 4, 8 and 16 μmol/L, respectively, as the low, medium, and high concentration groups of acacetin, and cells without any treatment were used as control groups; pcDNA, pcDNA-LZTFL1 was transfected into A549 cells and recorded as pcDNA group and pcDNA-LZTFL1 group; si-NC and si-LZTFL1 were transfected into A549 cells and treated with 16 μmol/L acacetin and recorded as acacetin+si-NC group, acacetin+si-LZTFL1 group. Tetramethylazozolium colorimetry (MTT) was used to detect cell proliferation inhibition rate; Western blotting was used to detect LZTFL1, CyclinD1, p21, MMP-2, and MMP-9 protein expression; Transwell was used to detect cell migration and invasion; real-time fluorescent quantitative PCR (RT-qPCR) was used to detect the expression of LZTFL1 mRNA. Results Compared with the control group, the cell proliferation inhibition rate of lung cancer A549 cells treated with low, medium, and high concentrations of acacetin was significantly increased, the number of cell migration and invasion was significantly reduced, and the expressions of CyclinD1, MMP-2 and MMP-9 proteins were significantly reduced, the expression of p21 protein was significantly increased, LZTFL1 mRNA and protein expression levels were significantly increased (P < 0.05). LZTFL1 overexpression inhibits the proliferation, migration and invasion of lung cancer cells. Interfering with LZTFL1 expression reversed the inhibitory effect of acacetin on the proliferation, migration and invasion of lung cancer A549 cells. Conclusions Acacetin may inhibit the proliferation, migration and invasion of lung cancer cells by up-regulating the expression of LZTFL1.

R965