DNA加合物是評價DNA損傷最直接的分子證據(jù)，隨之產(chǎn)生一個專門研究DNA修飾識別和DNA加合物定量的新領(lǐng)域——DNA加合物組學。其中質(zhì)譜（MS）分析法是常用的DNA加合物組學技術(shù)，并且隨著質(zhì)譜分析儀的靈敏度和掃描速度的提高，使得利用DNA加合物組學技術(shù)來篩查人類基因組中DNA損傷成為可能。其中，基于質(zhì)譜技術(shù)對馬兜鈴、巴豆醛等致癌物生成的DNA加合物的種類的檢測應用十分廣泛。主要就基于質(zhì)譜的加合物組學檢測技術(shù)及其在致癌物檢測中的應用進行概述。

DNA adducts are the most direct molecular evidence for evaluating DNA damage, and a new field specializing in DNA modification recognition and DNA adduct quantification—DNA adductomics has emerged.Mass spectrometry (MS) is a commonly used DNA adductomics technology, and with the increase in the sensitivity and scanning speed of mass spectrometry, the use of DNA adductomics technology to screen DNA in the human genome Damage becomes possible. The detection of DNA adducts produced by carcinogens such as aristolochia and crotonaldehyde based on mass spectrometry is very widely used. This article mainly summarizes the adductomics detection technology based on mass spectrometry and its application in the detection of carcinogens.

R917

國家“重大新藥創(chuàng)制”科技重大專項（2018ZX09201017-001）；中國食品藥品檢定研究院學科帶頭人培養(yǎng)基金資助項目（2019X4）