目的 研究治咳川貝枇杷滴丸（CBPP）的祛痰、鎮(zhèn)咳作用，以及對肺組織Muc5AC表達的影響，明確其藥效特點，為中藥臨床定位提供實驗依據(jù)。方法 采用雄性SPF級ICR小鼠，分別為對照（生理鹽水）組、鹽酸氨溴索口服液（陽性藥，臨床等效劑量11.7 mg/kg）組和CBPP低、中、高劑量（70.2、140.4、280.8 mg/kg）組，采用氣管酚紅排泌實驗結合分光光度計法探究CBPP的祛痰藥效；利用氨水引咳實驗觀察CBPP高劑量組的鎮(zhèn)咳作用；采用實時熒光定量PCR（qRT-PCR）法檢測CBPP高劑量組對肺組織中Muc5AC mRNA表達的影響。家兔隨機分為對照組、鹽酸氨溴索口服液（10.5 mg/mL）組、CBPP（252 mg/mL）組，利用離體氣管纖毛運動實驗考察CBPP對氣管纖毛運動的影響。結果 酚紅排泌實驗顯示，與對照組比較，陽性藥和CBPP顯著促進正常小鼠氣管酚紅排泌量增加（P<0.05、0.01），且CBPP作用呈劑量相關性；家兔離體氣管纖毛運動實驗顯示，與對照組比較，陽性藥和CBPP顯著促進纖毛運動（P<0.05），進而促進痰液排出；氨水引咳實驗顯示，與對照組比較，CBPP顯著延長小鼠的咳嗽潛伏期、減少咳嗽次數(shù)（P<0.05）；qRT-PCR結果顯示，與對照組比較，CBPP顯著降低肺組織中Muc5AC的mRNA表達（P<0.05）。結論 CBPP具有明顯的祛痰作用，促進呼吸道腺體的分泌，并促進氣管纖毛運動，降低Muc5AC的含量，促進痰液排出，并且對氨水刺激引起的咳嗽有一定的鎮(zhèn)咳作用。

Objective To study the expectorant and antitussive effects of Zhike Chuanbei Pipa Dropping Pills (CBPP), and its effect on the expression of MUC5AC in lung tissue, clarify their pharmacodynamics characteristics, and provide experimental basis for the clinical positioning of this Chinese patent medicine. Methods Male SPF ICR mice were randomly divided into control group, positive drug group (ambroxol hydrochloride oral solution, 11.7 mg/kg), and CBPP low, medium and high-dose (70.2, 140.4, 280.8 mg/kg) group. Observe the expectorant effects of CBPP by phenol red expectorant experiment combined spectrophotometer method in normal mice. Rabbits were randomly divided intocontrol group, positive drug group (ambroxol hydrochloride oral solution,10.5 mg/mL) and CBPP (252 mg/mL) group. The rabbit in vitro tracheal ciliary exercise experiment was used to examine the effect of CBPP on tracheal cilia movement. The antitussive effect of CBPP of high dose by using normal mice with ammonia water induced cough experiment. Detect the content of Muc5AC mRNA in lung tissue by qRT-PCR in control and CBPP high dose group. Results Phenol red excretion experiments showed that compared with control group, the positive drug and CBPP promoted the increase of tracheal phenol red excretion in mice (P<0.05 or 0.01), and promoted the secretion of respiratory tract glands in mice, and CBPP had a certain dose-dependent effect. Rabbit in vitro tracheal ciliary exercise experiment showed treatment of positive drug and high dose of CBPP promoted cilia movement (P<0.05), and then promoted sputum excretion. Ammonia water-induced cough experiment showed that compared with control group, CBPP high-dose group prolonged the cough latency period of mice and reduced the number of coughs (P<0.05). qRT-PCR results showed that compared with control group, CBPP administration reduced the content of Muc5AC mRNA in lung tissue (P<0.05). Conclusion CBPP has obvious expectorant effect, promotes the secretion of respiratory tract glands, and promotes the movement of tracheal cilia, reduces the content of Muc5AC, promotes sputum discharge, and has a certain antitussive effect on cough caused by ammonia stimulation.

R285.5

十三五天津市高等學?！皠?chuàng)新團隊培養(yǎng)計劃”基金資助項目（TD13-5050）