目的 評價二甲基姜黃素、黃芩苷、紫檀茋對異煙肼導(dǎo)致斑馬魚神經(jīng)損傷的保護作用。方法 挑選受精后2 h（2hpf）的斑馬魚胚胎，設(shè)置對照組（胚胎培養(yǎng)用水處理）、異煙肼組（4 mmol/L）、二甲基姜黃素（0.5、1.0、2.0、4.0 μmol/L）組、黃芩苷（0.1、0.2、0.5、1.0 μmol/L）組、紫檀茋（0.5、1.0、2.0、4.0 μmol/L）組，異煙肼分別與不同濃度的藥物共處理120 h，用Zebrabox斑馬魚行為分析儀分析斑馬魚幼魚行為學(xué)軌跡變化，熒光顯微鏡下觀察斑馬魚幼魚多巴胺神經(jīng)元的發(fā)育情況。結(jié)果 與對照組比較，異煙肼組運動軌跡明顯減少，運動總距離和低速、中速運動距離均顯著下降（P<0.01），運動速度明顯降低；神經(jīng)節(jié)長度顯著降低（P<0.01）。與異煙肼組比較，二甲基姜黃素組運動軌跡明顯增加，斑馬魚運動狀態(tài)主要為低速運動，各濃度的二甲基姜黃素組斑馬魚總運動距離和低速運動距離均顯著增加（P<0.01），運動速度明顯增加，0.5、2.0、4.0 μmol/L的二甲基姜黃素組中速運動距離顯著增加（P<0.01）；黃芩苷組運動軌跡明顯增加，0.1、0.2、0.5和1.0 μmol/L的黃芩苷組斑馬魚總運動距離和低速運動距離均顯著增加（P<0.01），運動速度明顯增加；0.5和1.0 μmol/L紫檀茋組運動軌跡明顯增加，紫檀茋0.5 μmol/L組的總運動距離和低速運動距離均顯著增加（P<0.01），紫檀茋0.5和1.0 μmol/L濃度組運動速度明顯增加。與異煙肼組比較，二甲基姜黃素和黃芩苷各濃度組多巴胺相對神經(jīng)節(jié)長度顯著增加（P<0.01）；紫檀茋1 μmol/L組相對神經(jīng)節(jié)長度顯著增加（P<0.01）。結(jié)論 二甲基姜黃素、黃芩苷和0.5、1.0 μmol/L紫檀茋對異煙肼導(dǎo)致的斑馬魚神經(jīng)損傷有明顯保護作用。

Objective To evaluate the protective effects of dimethyl curcumin, baclidin and pterostilbene on isoniazid induced nerve damage in zebrafish. Methods 2 hpf (hours post fertilization) zebrafish embryos were used in the experiments. These fish were respectively treated with control group, isoniazid group, and isoniazid along with different concentrations of dimethyl curcumin (0.5, 1.0, 2.0, 4.0 μmol/L), bacitrin (0.1, 0.2, 0.5, 1.0 μmol/L) and pterostilbene (0.5, 1.0, 2.0, 4.0 μmol/L) co-treatment group. At 120 h, the Zebrabox was used to analyze the behavioral trajectory changes of zebrafish, and the development of dopaminergic neurons in zebrafish larvae was observed under a fluorescence microscope. Results Compared with control group, the movement track of isoniazid group was significantly reduced, the total movement distance and the low and medium speed movement distance were significantly decreased (P < 0.01), and the movement speed was significantly reduced, and the ganglion length was significantly decreased (P < 0.01). Compared with isoniazid group, the movement track of dimethylcurcumin group was significantly increased, and the zebrafish movement state was mainly low speed movement. The total movement distance and low speed movement distance of each concentration of dimethylcurcumin group were significantly increased (P < 0.01), and the movement speed was significantly increased, the distance of middle speed movement was significantly increased in dimethylcurcumin 0.5, 2.0, 4.0 μmol/L group (P < 0.01). In the baicalin group, the trajectories increased significantly, the total movement distance and low speed movement distance of zebrafish were significantly increased (P < 0.01) and the movement speed was significantly increased (P < 0.01). The trajectories increased significantly in pterostilbene 0.5 and 1.0 μmol/L group, the total movement distance and low speed movement distance of pterostilbene 0.5 μmol/L group were significantly increased (P < 0.01) and the movement speed was significantly increased in pterostilbene 0.5 and 1.0 μmol/L group. Compared with isoniazid group, the relative ganglion length of dopamine in dimethylcurcumin, baicalin and pterostilbene 1 μmol/L groups was significantly increased (P < 0.01).Conclusion Dimethylcurcumin, baclidin and lower-concentration pterostilbene have obvious protective effects on isoniazid-caused zebrafish nerve injury.

山東省優(yōu)秀青年人才基金項目（ZR2020YQ60）；齊魯工業(yè)大學(xué)（山東省科學(xué)院）科教產(chǎn)融合創(chuàng)新試點工程項目（2020KJCZD08）；濟南市“高校20條”資助項目（2020GXRC053）