目的 以斑馬魚為模型，評價(jià)三峽陽菊葉水提物（WELTGY）對斑馬魚整體形態(tài)以及心臟、腎臟和肝臟等主要臟器的影響。方法 用不同濃度（2.8、3.4、3.8、4.8、5.8、6.8 mg/mL）的WELTGY處理受精后72 h（72 hpf）的斑馬魚，處理24、48 h后，觀察斑馬魚死亡率、畸形率和整體形態(tài)；顯微鏡下觀察斑馬魚心臟形態(tài)并拍照，記錄心率，使用ImagePro Plus 5.1測量斑馬魚靜脈竇-動(dòng)脈球（SV-BA）距離，評價(jià)WELTGY對斑馬魚心臟的影響；顯微鏡下觀察拍照記錄各組斑馬魚是否有眼水腫，判斷WELTGY對斑馬魚腎臟的影響；用WELTGY處理肝臟標(biāo)記綠色熒光的轉(zhuǎn)基因斑馬魚Tg（LFABP：EGFP），熒光顯微鏡下拍照記錄斑馬魚肝臟熒光情況，評價(jià)WELTGY對斑馬魚肝臟的影響。結(jié)果 與對照組比較，2.8 mg/mL及以下濃度的WELTGY對斑馬魚的整體形態(tài)、心臟、腎臟和肝臟無明顯影響；3.4 mg/mL以上濃度的WELTGY導(dǎo)致斑馬魚魚鰾減小，對心臟、肝臟、腎臟無影響；4.8 mg/mL以上濃度的WELTGY導(dǎo)致斑馬魚心率顯著降低（P<0.01），對心臟SV-BA距離、腎臟、肝臟熒光強(qiáng)度和熒光面積無明顯影響。結(jié)論 WELTGY毒性微弱，對斑馬魚幼魚幾乎沒有毒性；高濃度下會(huì)導(dǎo)致輕微心臟毒性，在家禽養(yǎng)殖業(yè)的使用中有必要合理控制用量。

Objective To evaluate the effects of water extract from leaves of Three Gorges Yangju (WELTGY) on the development of morphology and main organs such as heart, kidney and liver by zebrafish model. Methods Zebrafish embryos at 72 hpf (hours post fertilization) were treated with different concentrations (2.8, 3.4, 3.8, 4.8, 5.8, and 6.8 mg/mL) of WELTGY. The mortality rate, malformation rate and overall morphology of zebrafish were observed after treatment for 24 and 48 h. The heart morphology of zebrafish was observed and photographed under a microscope, and the heart rate was recorded. Image-Pro Plus 5.1 was used to measure the distance between the venous sinuso-artery bulb (SV-BA) of zebrafish, so the effect of WELTGY on the heart of zebrafish was evaluated. The effect of WELTGY on the kidney of zebrafish was determined by observing and photographing zebrafish eye edema in each group under microscope. The liver labeled green fluorescent transgenic zebrafish Tg(L-FABP:EGFP) was treated with WELTGY, and the liver fluorescence of zebrafish was photographed under fluorescence microscope to evaluate the effect of WELTGY on the liver of zebrafish. Results WELTGY at a concentration of 2.8 mg/mL or less had no effect on the overall morphology, heart, liver and kidney of zebrafish. Concentrations above 3.4 mg/mL caused the swim bladder to decrease, and had no effects on the heart, kidney, and liver. Concentrations above 4.8 mg/mL caused a significant decrease in zebrafish heart rate, and had no effects on the distance between the heart venous sinus -bulus arteriosus, kidney, liver fluorescence intensity, and fluorescence area. Conclusion In this study, WELTGY was found to be weakly toxic and had almost no effects on zebrafish embryogenesis. High concentrations could cause slight cardiotoxicity. It is necessary to strengthen the management and control the dosage in the use of poultry breeding.

R965.3

山東省技術(shù)創(chuàng)新引導(dǎo)計(jì)劃（魯渝科技協(xié)作）項(xiàng)目（2020LYXZ017）；重慶市科技局魯渝科技合作項(xiàng)目（CSTC2020JSCX-LYJ‐SA0030）；山東省優(yōu)秀青年人才基金項(xiàng)目（ZR2020YQ60）；齊魯工業(yè)大學(xué)（山東省科學(xué)院）科教產(chǎn)融合創(chuàng)新試點(diǎn)工程項(xiàng)目（2020KJC-ZD08）