目的 基于偏最小二乘回歸法（PLS）研究梔子不同炮制品化學(xué)成分與肝腎毒性的關(guān)聯(lián)度。方法 采用超高效液相色譜法測(cè)定生梔子、炒梔子、姜梔子、焦梔子、梔子炭不同炮制品中梔子苷、京尼平龍膽雙糖苷、梔子苷酸、去乙酰車葉草酸甲酯、西紅花苷I和西紅花苷Ⅱ 6種化學(xué)成分的含量，梔子不同炮制品水提液（7.5 g/kg）連續(xù)3 d大鼠ig給藥，測(cè)定各組大鼠血清丙氨酸氨基轉(zhuǎn)移酶（ALT）、天冬氨酸氨基轉(zhuǎn)移酶（AST）、總膽紅素（TBIL）、血清肌酐（CREA）、尿素氮（BUN）的含量，使用Origin 9.1軟件中的PLS算法分析梔子不同炮制品化學(xué)成分與肝腎毒性的關(guān)聯(lián)度。結(jié)果 梔子炮制后梔子苷、西紅花苷I和西紅花苷Ⅱ的含量不同程度的降低，京尼平龍膽雙糖苷、去乙酰車葉草酸甲酯和梔子苷酸含量在部分炮制品中升高；梔子及炮制品對(duì)正常大鼠可造成不同程度的肝、腎損傷（生梔子>炒梔子>姜梔子>焦梔子>梔子炭）；PLS分析表明6種主要化學(xué)成分中梔子苷與西紅花苷I的變量重要性值均超過(guò)0.8。結(jié)論 梔子苷與西紅花苷I可能是梔子肝腎毒性的物質(zhì)基礎(chǔ)，通過(guò)炮制降低梔子肝腎毒性的作用可能與炮制后梔子苷與西紅花苷I含量降低有關(guān)。

Objective To explore the relationship between the chemical constituents and liver and kidney toxicity of different processed Gardenia jasminoides products based on partial least square regression. Methods The contents of chemical constituents in 5 processed products of Gardenia jasminoides were determined by ultra performance liquid chromatography, water extracts of different processed Gardenia jasminoides products (7.5 g/kg) were administered by gavage to rats for three consecutive days, and the contents of ALT, AST, TBIL, CREA, and BUN in serum were determined at the last day, the correlation between chemical constituents and liver and kidney toxicity of different processed Gardenia jasminoides products were analyzed using the PLS algorithm in the Origin 9.1 software. Results The contents of geniposide, crocetin I and crocetin II decreased to varying degrees after processed, but the contents of genipin-1-β-D-gentiobioside, geniposidic acid,deacetyl asperulosidic acid methyl esterin were higher than those in raw Gardenia jasminoides. Five kinds of processed Gardenia jasminoides products caused different liver and kidney damage on normal rats (raw Gardenia jasminoides > fried Gardenia jasminoides > ginger Gardenia jasminoides > scorched Gardenia jasminoides > Gardenia jasminoides charcoal). PLS analysis showed the variable importance values of geniposide and crocetin I among the six main chemical components exceed 0.8. Conclusion Geniposide and crocetin I may be the material basis of Gardenia jasminoides which caused liver and kidney toxicity. The effect of reducing liver and kidney toxicity caused by processing may be related to the decrease of the contents geniposide and crocetin I.

R283.1

國(guó)家自然科學(xué)基金（81903913）