目的 運(yùn)用細(xì)胞膜色譜（CMC）技術(shù)檢測(cè)分析人參皂苷與五味子木脂素中15種成分與血管內(nèi)皮生長(zhǎng)因子（VEGF）受體的作用。方法 運(yùn)用Western blotting法檢測(cè)篩選VEGF受體高表達(dá)細(xì)胞株；構(gòu)建VEGF受體CMC，并建立ECV304/CMC online-LC檢測(cè)方法，以索拉菲尼作為陽(yáng)性藥，對(duì)人參皂苷Rb₁、Rb₂、Rb₃、Rc、Rd、Rg₁、Rg₂、Rg₃、Rh₁、Ro、F₂，五味子甲素，五味子乙素，五味子醇甲，五味子醇乙進(jìn)行VEGF受體結(jié)合強(qiáng)度的檢測(cè)分析；CCK-8法檢測(cè)篩選出的成分（5、10 μg/mL）對(duì)ECV304細(xì)胞的促增殖作用。結(jié)果 Western blotting結(jié)果顯示，ECV304細(xì)胞中VEGF受體高表達(dá)；人參皂苷Rb₁、Rb₂、Rb₃、Rc、Rd、Rg₃、Rh₁、F₂、五味子甲素、五味子乙素與VEGF受體CMC柱有結(jié)合作用；CCK-8法檢測(cè)以上成分對(duì)ECV304細(xì)胞的活力影響發(fā)現(xiàn)，人參皂苷Rb₂能促進(jìn)細(xì)胞的增殖。結(jié)論 人參皂苷Rb₂能與VEGF受體結(jié)合，且顯著促進(jìn)ECV304細(xì)胞增殖。

Objective To detect and analyze the effect of 15 components from ginseng saponins and schisandra lignin on angiogenesis by cell membrane chromatography. Methods Western blotting detection was used to screen the cell lines with high expression of VEGF receptor. Cell membrane chromatographic column was prepared by ECV304 cell membrane and ECV304/CMC online-LC detection method was established. With sorafenib as a positive drug, the receptor binding strength of VEGF was detected and analyzed for ginsenosides Rb₁,Rb₂,Rb₃,Rc,Rd,Rg₁,Rg₂,Rg₃,Rh₁,Ro,F₂, deoxyschizandrin, γ -schizandrin, schisandrol A, schisandrol B. CCK-8 assay was used to detect the proliferative effects of the selected ingredients (5 and 10 μg/mL) on ECV304 cells. Results Western blotting results showed that VEGF receptor was highly expressed in ECV304 cells. It was found that ginsenoside Rb₁,Rb₂,Rb₃,Rc,Rd,Rg₃,Rh₁,F₂, schisandrin A and schisandrin B had binding effect. CCK-8 assay showed that ginsenoside Rb₂ could promote the proliferation of ECV304 cells. Conclusion Ginsenoside Rb₂ can bind to angiogenic VEGF receptor, and can significantly promote the proliferation of ECV304.

R285.5