目的 基于文本挖掘技術(shù)和生物醫(yī)學(xué)數(shù)據(jù)庫(kù)對(duì)新型冠狀病毒肺炎（COVID-19）相關(guān)文獻(xiàn)進(jìn)行數(shù)據(jù)挖掘分析，探究COVID-19及其主要癥狀發(fā)熱、咳嗽、呼吸障礙相關(guān)基因靶點(diǎn)，篩選潛在有效的化學(xué)藥和中藥。方法 使用GenCLiP 3網(wǎng)站獲取COVID-19和其主要癥狀咳嗽、發(fā)熱、呼吸障礙共4個(gè)關(guān)鍵詞的共有靶點(diǎn)，在METASCAPE數(shù)據(jù)庫(kù)中對(duì)其進(jìn)行基因本體（GO）和通路富集分析，再利用String數(shù)據(jù)庫(kù)和Cytoscape軟件構(gòu)建共有靶點(diǎn)的蛋白質(zhì)相互作用網(wǎng)絡(luò)，篩選獲得核心基因，運(yùn)用DGIdb數(shù)據(jù)庫(kù)、SymMap數(shù)據(jù)庫(kù)針對(duì)核心基因進(jìn)行中西醫(yī)治療藥物預(yù)測(cè)。結(jié)果 獲得COVID-19及其主要癥狀共有基因靶點(diǎn)28個(gè)，其中有IL2、IL1B、CCL2等核心基因16個(gè)，使用DGIdb數(shù)據(jù)庫(kù)篩選獲得與16個(gè)關(guān)鍵靶點(diǎn)相互作用的化學(xué)藥包括沙利度胺、來(lái)氟米特、環(huán)孢素等28種，中藥包括虎杖、黃芪、蘆薈等70味。結(jié)論 COVID-19及其主要癥狀的病理機(jī)制可能和CD4、KNG1、VEGFA等28個(gè)共有基因相關(guān)，可能通過(guò)介導(dǎo)TNF、IL-17等信號(hào)通路參與COVID-19病理過(guò)程。潛在有效藥物可能通過(guò)作用相關(guān)靶點(diǎn)通路起到治療COVID-19的作用。

Objective Based on text mining technology and biomedical database, data mining and analysis of coronavirus disease 2019 (COVID-19) were carried out, and COVID-19 and its main symptoms related to fever, cough and respiratory disorders were explored. Methods The common targets of COVID-19 and its main symptoms cough, fever and respiratory disorder were obtained by GenCLiP 3 website, Gene ontology in metascape database (GO) and pathway enrichment analysis, then STRING database and Cytoscape software were used to construct the protein interaction network of common targets, the core genes were screened and obtained. DGIdb database and Symmap database were used to predict the therapeutic drugs of traditional Chinese and Western medicine for the core genes. Results A total of 28 gene targets of COVID-19 and its main symptoms were obtained, including 16 core genes such as IL2, IL1B and CCL2. Through the screening of DGIdb database, 28 chemicals interacting with 16 key targets were obtained, including thalidomide, leflunomide and cyclosporine et al. And 70 kinds of Chinese meteria medica including Polygonum cuspidatum, Astragalus membranaceus and aloe. Conclusion The pathological mechanism of COVID-19 and its main symptoms may be related to 28 common genes such as CD4, KNG1 and VEGFA, which may participate in the pathological process of COVID-19 by mediating TNF, IL-17 and other signal pathways. Potentially effective drugs may play a role in the treatment of COVID-19 through action related target pathway.

R965.1

國(guó)家科技重大專項(xiàng)課題資助項(xiàng)目（2018ZX10303-502-003）；國(guó)家重點(diǎn)研發(fā)計(jì)劃中醫(yī)藥現(xiàn)代化研究重點(diǎn)專項(xiàng)（2018YFC1705702）；京津冀中醫(yī)國(guó)家臨床重點(diǎn)?？聘尾f(xié)同病房項(xiàng)目（GZY-GCS-2017-60）