目的 探究蓽鈴胃痛顆粒對乙醇誘導(dǎo)胃潰瘍模型大鼠的保護作用。方法 SD大鼠隨機分為對照組、模型組及蓽鈴胃痛顆粒低、中、高劑量（0.79、1.58、3.16 g·kg^-1）組和西咪替丁（42.00 mg·kg^-1，陽性藥）組，每組8只；各組均按劑量預(yù)給藥8 d，對照組和模型組大鼠ig等體積0.5%羧甲基纖維素鈉（CMC-Na）溶液。末次給藥30 min后，除對照組外，其余大鼠ig給予1 mL無水乙醇造模，1 h后犧牲動物取材；展開胃黏膜面拍照，測量潰瘍面積，取部分胃組織進行HE染色；檢測血清中白細胞介素-1β（IL-1β）、腫瘤壞死因子-α（TNF-α）和超氧化物歧化酶（SOD）水平，檢測胃組織中髓過氧化物酶（MPO）和前列腺素E₂（PGE₂）水平。結(jié)果 與對照組比較，模型組大鼠胃潰瘍面積和胃黏膜病理評分顯著升高（P<0.001），大鼠血清中IL-1β（P<0.01）和TNF-α（P<0.05）水平均顯著升高，大鼠胃組織MPO活力顯著升高（P<0.001），大鼠胃組織中PGE₂水平顯著降低（P<0.01）。與模型組比較，各給藥組大鼠胃潰瘍面積和胃黏膜病理評分顯著降低（P<0.05、0.01、0.001）。與模型組比較，蓽鈴胃痛顆粒組大鼠血清IL-1β和TNF-α水平顯著降低（P<0.05、0.001），胃組織中MPO活力顯著降低（P<0.01、0.001），血清SOD活力顯著升高（P<0.05）；胃組織PGE₂水平顯著增加（P<0.05）。結(jié)論 蓽鈴胃痛顆?？赏ㄟ^抑制炎癥因子分泌、緩解機體氧化應(yīng)激、保護胃黏膜等發(fā)揮對胃潰瘍模型大鼠的保護作用。

Objective To explore the protective effect of Biling Weitong Granules on ethanol-induced gastric ulcer rats. Methods Sprague Dawley rats were randomly divided into six groups: control group, model group, cimetidine group (42.00 mg·kg^-1, positive drug) and Biling Weitong Granules low-, middle- and high-dose group (0.79, 1.58 and 3.16 g·kg^-1), eight mice in each group. The drugs were given by intragastric administration before the model was established, once a day for eight days, while the rats in the control group and model group were given 0.5% carboxymethylcellulose sodium (CMC-Na) solution by intragastric administration. Thirty minutes after the last administration, excluding rats in the control group, the rest of the rats were given 1 mL of absolute ethanol by intragastric administration to induce gastric ulcers. One hour later, all rats were sacrificed immediately. The gastric mucosal surface was unfolded to take pictures and the area of gastric ulcers was measured. HE staining of stomach sections were carried out to observe pathological changes. Interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), total superoxide dismutase (SOD), myeloperoxidase (MPO) and prostaglandin E₂ (PGE₂) were detected. Results Compared with the control group, the area of gastric ulcer and pathological score of gastric mucosa in the model group increased significantly (P < 0.001), the levels of IL-1β (P < 0.01) and TNF-α (P < 0.05) in serum, MPO activity (P < 0.001) in gastric tissue increased significantly, and the level of PGE₂ ingastric tissue decreased significantly (P < 0.01). Compared with the model group, the area of gastric ulcer and pathological score of gastric mucosa in each treatment group were significantly lower (P < 0.05, 0.01, 0.001). Compared with the model group, the levels of serum IL-1β and TNF-α in Biling Weitong Granule group were significantly lower (P < 0.05, 0.001), the activity of MPO in gastric tissue was significantly lower (P < 0.01, 0.001), and the activity of serum SOD was significantly higher (P < 0.05), the level of PGE₂ in gastric tissue increased significantly (P < 0.05). Conclusion Biling Weitong Granule can protect gastric ulcer model rats by inhibiting the secretion of inflammatory factors, alleviating oxidative stress and protecting gastric mucosa.

R285.5

泰州市重大科技成果轉(zhuǎn)化項目（SCG201805）