目的 探討羅布麻葉水提物（water extract of Apocynum venetum leaves，AVLWE）鎮(zhèn)靜催眠作用及機(jī)制。方法 隨機(jī)將ICR小鼠分為對(duì)照組、右佐匹克?。栃运?，0.40 mg·kg^-1）組和AVLWE低、中、高劑量（0.58、1.17、2.34 g·kg^-1）組，每天ig給藥1次，連續(xù)4周，每周稱體質(zhì)量；于末次給藥前1 d給藥60 min后，應(yīng)用曠場(chǎng)視頻分析系統(tǒng)檢測(cè)各組小鼠5 min自主活動(dòng)；于末次給藥45 min后，各組動(dòng)物ip 1%戊巴比妥鈉（35 mg·kg^-1，閾下催眠劑量），記錄30 min內(nèi)入睡潛伏期、入睡動(dòng)物數(shù)、睡眠時(shí)間；結(jié)束后麻醉處死動(dòng)物，取腦稱質(zhì)量并計(jì)算腦系數(shù)。SD大鼠分為對(duì)照組、模型組、右佐匹克?。栃运帲?.27 mg·kg^-1）組和AVLWE低、中、高劑量（0.40、0.81、1.62 g·kg^-1）組，每天ig給藥1次，連續(xù)4周，每周稱體質(zhì)量；除對(duì)照組外，給藥第28、29天ip對(duì)氯苯丙氨酸（PCPA）制備大鼠失眠模型，給藥結(jié)束稱取腦質(zhì)量并計(jì)算腦系數(shù)，取下丘腦，ELISA試劑盒法測(cè)定5-羥色胺（5-HT）、多巴胺（DA）和5-羥吲哚乙酸（5-HIAA）的含量。結(jié)果 小鼠實(shí)驗(yàn)結(jié)果表明，與對(duì)照組比較，各給藥組第1~4周體質(zhì)量均顯著降低（P<0.01）；右佐匹克隆片和AVLWE中、高劑量組睡眠發(fā)生率均顯著增加（P<0.05、0.01）；右佐匹克隆片和AVLWE中、高劑量組睡眠時(shí)間顯著延長(zhǎng)（P<0.05）；AVLWE低、中、高劑量組腦系數(shù)均顯著升高（P<0.01）。大鼠實(shí)驗(yàn)結(jié)果表明，與對(duì)照組比較，AVLWE高劑量組第1~4周體質(zhì)量均顯著降低（P<0.05、0.01）；與模型組比較，AVLWE高劑量組腦系數(shù)顯著升高（P<0.05）；右佐匹克隆片組、AVLWE高劑量組DA水平顯著降低（P<0.05）、5-HIAA水平顯著升高（P<0.01），AVLWE低、中、高劑量組5-HT水平顯著升高（P<0.05、0.01）。結(jié)論 AVLWE具有改善睡眠的作用，機(jī)制可能與上調(diào)下丘腦5-HT水平、下調(diào)DA水平有關(guān)。

Objective To explore the sedative and hypnotic effects of water extract of Apocynum venetum leaves (AVLWE) and its preliminary mechanism. Methods ICR mice were randomly divided into control group, dexzopicron (positive drug, 0.40 mg·kg^-1) group and AVLWE low-dose, medium-dose and high-dose groups (0.58, 1.17, 2.34 g·kg^-1), ig once a day for four weeks, and body weight was weighed weekly. After 60 min of administration one day before the last administration, open field video analysis system was used to detect 5 min autonomous activities of mice in each group. 45 min after the last administration, animals in each group were given a subthreshold hypnotic dose of 1% pentobarbital sodium (35 mg·kg^-1), and the sleep latency, number of sleeping animals and sleep time within 30 min were recorded. After anesthesia, the animals were killed, the brain was weighed and the brain coefficient was calculated. SD rats were divided into control group, model group, dexzopicron (positive drug, 0.27 mg·kg^-1) group and AVLWE low-dose, medium-dose and high-dose groups (0.40, 0.81, 1.62 g·kg^-1), ig once a day for four weeks, and body weight was weighed weekly. Except for the control group, the insomnia model of rats was prepared by ip p-chlorophenylalanine (PCPA) on the 28th and 29th day of administration. At the end of administration, the brain weight was measured and the brain coefficient wascalculated. The hypothalamus was taken and the contents of 5-hydroxytryptamine (5-HT), dopamine (DA) and 5- hydroxyindoleacetic acid (5-HIAA) were determined by ELISA kit method. Results The results of mouse experiment showed that compared with the control group, the body weight of each administration group was significantly decreased from week one to four (P < 0.01). The incidence of sleep in dexzopiclone tablets and AVLWE medium and high dose groups was significantly increased (P < 0.05, 0.01); Sleep time of dexzopiclone tablets and AVLWE medium and high dose groups was significantly prolonged (P < 0.05); The brain coefficients of AVLWE low-dose, medium-dose and high-dose groups were significantly increased (P < 0.01). Experimental results in rats showed that, compared with control group, body weight of AVLWE high-dose group was significantly decreased from week one to four (P < 0.05, 0.01). Compared with model group, brain coefficient of AVLWE high-dose group was significantly increased (P < 0.05); DA was significantly decreased (P < 0.05) and 5-HIAA was significantly increased (P < 0.01) in dexzopiclone tablet group and high dose AVLWE group, while 5-HT was significantly increased (P < 0.05, 0.01) in low, medium and high dose AVLWE groups. Conclusion AVLWE has the effect of improving sleep. The mechanism of action may be related to the content of 5-HT and DA.

R285.5

新疆維吾爾自治區(qū)重大科技專項(xiàng)項(xiàng)目（2016A03006，2016A03006-2，2016A03006-4）