目的 研究余甘子Phyllanthus emblica L.鞣質(zhì)對(duì)代謝相關(guān)脂肪性肝病（MAFLD）小鼠脂質(zhì)代謝及腸道菌群的調(diào)節(jié)作用。方法 將C57BL/6小鼠隨機(jī)分為對(duì)照組、模型組和余甘子鞣質(zhì)低、高劑量（200、400 mg·kg^-1）組及非諾貝特（陽(yáng)性藥，50 mg·kg^-1）組，每組10只。對(duì)照組小鼠常規(guī)飼料喂養(yǎng)，其余各組小鼠均給予高脂飼料喂養(yǎng)，建立MAFLD小鼠模型；ig給予相應(yīng)藥物干預(yù)，對(duì)照組及模型組給予同體積生理鹽水，每天1次，連續(xù)給藥8周。處死小鼠，收集血液、肝臟、糞便。計(jì)算肝臟系數(shù)；HE染色法觀察肝組織病理學(xué)變化；試劑盒法檢測(cè)小鼠血清中丙氨酸氨基轉(zhuǎn)移酶（ALT）、天冬氨酸氨基轉(zhuǎn)移酶（AST）、三酰甘油（TG）、總膽固醇（TC）含量；實(shí)時(shí)熒光定量PCR （qRT-PCR）及Western blotting法檢測(cè)肝組織中固醇調(diào)節(jié)元件結(jié)合蛋白（SREBP-1）、脂肪酸合成酶（FASN）、乙酰輔酶A羧化酶（ACC） mRNA和蛋白表達(dá)量；PCR擴(kuò)增法檢測(cè)實(shí)驗(yàn)前后小鼠糞便雙歧桿菌、乳酸桿菌的含量變化。結(jié)果 與模型組比較，低、高劑量余甘子鞣質(zhì)干預(yù)后小鼠肝臟系數(shù)顯著降低（P<0.05、0.01），小鼠肝組織脂肪變程度減輕；小鼠血清中ALT、AST、TG、TC水平均顯著降低（P<0.05、0.01）；肝組織中SREBP-1、FASN、ACC mRNA和蛋白水平均顯著降低（P<0.05、0.01）；糞便中雙歧桿菌、乳酸桿菌的含量顯著升高（P<0.01）。結(jié)論 余甘子鞣質(zhì)能夠通過(guò)SREBP-1/FASN/ACC通路調(diào)節(jié)脂質(zhì)代謝、改善腸道菌群平衡，改善MAFLD。

Objective To study the effect of tannin part of Phyllanthus emblica on fatty acid metabolism and intestinal flora in metabolic associated fatty liver disease（MAFLD) mice. Methods C57BL/6 mice were randomly divided into control group, model group, low and high dose (200 and 400 mg·kg^-1) tannin part of Phyllanthus emblica group and fenofibrate (positive, 50 mg·kg^-1) group, 10 rats in each group. Control mice fed normal diet, the other groups of mice were given a high fat diet to establish the MAFLD model. At the same time, tannin part of Phyllanthus emblica low and high dose drugs were given to the corresponding drug intervention, and the control group and model group were given the same volume saline. After eight weeks of continuous administration, mice were executed to collect blood, liver tissue and faeces samples. HE staining method was used to observe the pathological changes of liver tissue and the biochemical instrument was used to detect the content of ALT, AST, TG and TC in serum of mice. Western blotting and qRT-PCR were used to detect the expression of gene proteins such as SREBP-1, FASN and ACC in liver tissue, and qPCR to detect the number of Bifidobacterium and Lactobacillus in mouse faeces. Results Compared with model group, in low and high dose tannin part of Phyllanthus emblica group, the liver index in mice was significantly decreased (P < 0.05, 0.01), the degree of fat change in liver tissue was alleviated, the serum levels of ALT, AST, TG and TC were significantly decreased (P < 0.05, 0.01), mRNA and protein levels of SREBP-1, FASN andACC in liver tissue were significantly decreased (P < 0.05, 0.01), the contents of Bifidobacteria and Lactobacillus in feces were significantly increased (P < 0.01). Conclusion The tannin part of Phyllanthus emblica can lipid metabolism through SREBP-1/FASN pathway, and can regulate intestinal flora balance, which has a certain preventive effect on MAFLD.

R285.5

河南省科技發(fā)展計(jì)劃（152300410160）