目的 探討靈芝孢子油、破壁靈芝孢子粉及靈芝孢子提取物對小鼠急性胃潰瘍的影響。方法 雄性ICR小鼠隨機(jī)分為12組：對照組，模型組，奧美拉唑（陽性藥，4 mg·kg^-1）組，靈芝孢子提取物低、中、高劑量（0.2、0.4、0.8 g·kg^-1）組，破壁靈芝孢子粉低、中、高劑量（0.6、1.2、2.4 g·kg^-1）組，靈芝孢子油低、中、高劑量（0.2、0.4、0.8 g·kg^-1）組，每組20只。ig給藥，每天1次，對照組和模型組給予等體積的生理鹽水。連續(xù)給藥7 d后，禁食12 h，除對照組外，每組隨機(jī)選取10只小鼠ig無水乙醇（10 mL·kg^-1）建立急性胃潰瘍模型，1.5 h后取材；每組余下10只小鼠ig吲哚美辛（40 mg·kg^-1）建立急性胃潰瘍模型，3 h后取材。觀察小鼠的一般狀況、胃出血和潰瘍情況，進(jìn)行胃損傷評分，計算損傷抑制率和損傷發(fā)生率。結(jié)果 各組小鼠給藥過程中無異常。小鼠給予無水乙醇后，模型組和奧美拉唑組10 min后開始出現(xiàn)行動變緩，肢體活動不協(xié)調(diào)，呼吸加深、頻率減慢等癥狀，而給予靈芝孢子提取物、破壁靈芝孢子粉和靈芝孢子油的小鼠在30 min后才陸續(xù)出現(xiàn)癥狀；給予吲哚美辛的小鼠自主活動減少，各組之間無差別；對照組小鼠行為活動如常。與對照組比較，小鼠經(jīng)無水乙醇、吲哚美辛ig導(dǎo)致非常明顯的胃潰瘍，胃損傷評分顯著升高（P<0.01），損傷發(fā)生率為100%；與模型組比較，預(yù)防性給予靈芝孢子提取物、破壁靈芝孢子粉、靈芝孢子油可明顯降低胃出血或潰瘍等胃黏膜損傷，其中靈芝孢子提取物高劑量組、破壁靈芝孢子粉組、靈芝孢子油組的胃損傷評分顯著降低（P<0.01），損傷抑制率顯著提高（P<0.01），損傷發(fā)生率明顯降低。結(jié)論 靈芝孢子油、破壁靈芝孢子粉及靈芝孢子提取物能有效抑制小鼠急性胃潰瘍的發(fā)生，靈芝孢子油效果最佳，破壁靈芝孢子粉次之，并且對于無水乙醇導(dǎo)致的急性胃潰瘍抑制作用尤為顯著。

Objective To explore the influence of Ganoderma lucidum spores oil (GLSO), broken Ganoderma lucidum spore (BGLS) and Ganoderma lucidum spore extract (GLSE) on the formation of acute gastric ulcer. Methods Male ICR mice were randomly divided into 12 groups: control group, model group, omeprazole (4 mg·kg^-1) group, GLSE low, medium and high dose (0.2, 0.4, 0.8 g·kg^-1) groups, BGLS low, medium and high dose (0.6, 1.2, 2.4 g·kg^-1) groups, GLSO low, medium and high dose (0.2, 0.4, 0.8 g·kg^-1) groups, 20 in each group. Mice in control group and model group were given the same volume of normal saline. After seven days of continuous administration, ten mice in each group were randomly selected, except the control group, to establish acute gastric ulcer model intragastrically with anhydrous ethanol (10 mL·kg^-1), and the samples were taken 1.5 h later. Each group of the remaining 10 mice ig indomethacin (40 mg·kg^-1) to establish acute gastric ulcer models, the samples were collected after threehours. The general condition, gastric bleeding and ulcer of mice were observed, and the gastric injury score was performed, and the injury inhibition rate and injury incidence rate were calculated. Results There was no abnormality during administration in each group. After the mice were given anhydrous ethanol, the model group and omeprazole group began to show symptoms such as slowed movement, uncoordinated limb activity, deep breathing and slow frequency ten min later, while the mice given GLSO, BGLS and GLSE began to show symptoms 30 min later. The autonomic activity of indomethacin-induced model mice decreased in each group, and there was no difference among the groups. The behavior of mice in control group was as usual. Compared with control group, the gastric ulcer was induced by anhydrous ethanol and indomethacin intragastrically, and the gastric injury score was significantly increased (P < 0.01), and the injury rate was 100%. Compared with model group, preventive give GLSO, BGLS and GLSE can obviously reduce the bleeding or ulcer and gastric mucosa injury, stomach injury score significantly reduced (P < 0.01), the inhibition rate of damage increased significantly (P < 0.01), the injury rate was significantly reduced, in high dose group of GLSE, BGLS group and GLSO group. Conclusion BGLS and GLSE could suppress the formation of acute gastric ulcer in mice and the protective effect of GLSO on acute gastric ulcer was the best, followed by BGLS, and against significantly the alcoholic gastric ulcer.

R285.5