[關(guān)鍵詞]
[摘要]
目的 探討穿山龍復方水煎液對痛風性關(guān)節(jié)炎(GA)合并高尿酸血癥(HUA)誘導痛風性腎病(GN)大鼠腎臟的保護作用。方法 將48只雄性SD大鼠分為6組:對照組、模型組、秋水仙堿(陽性對照,0.03 mg·kg−1)組及穿山龍復方水煎液高、中、低劑量(6.300、3.150、1.575 mg·kg−1)組。各給藥組連續(xù)7 d ig 10 mL·kg−1相應藥液;對照、模型組各ig生理鹽水10 mL·kg−1。給藥過程中,除對照組外,其余建立GA合并HUA誘導的GN模型:每天2次ip 3%的氧嗪酸鉀溶液(10 mL·kg−1),連續(xù)1周;在大鼠右側(cè)踝關(guān)節(jié)后側(cè)沿跟腱內(nèi)側(cè)以30°~40°方向插入至關(guān)節(jié)腔,將0.2 mL尿酸鈉溶液(25 mg·mL−1)注入到關(guān)節(jié)腔內(nèi),以關(guān)節(jié)囊對側(cè)鼓起為注入標準,飼養(yǎng)過程中給予10%酵母飼料。稱雙側(cè)腎臟質(zhì)量,計算腎臟系數(shù);HE染色后觀察腎臟組織病理學變化;自動生化分析儀檢測大鼠血尿酸(SUA)、血肌酐(SCr)、血清尿素氮(BUN)水平以及尿液尿酸(UUA)、尿肌酐(UCr)水平;試劑盒法檢測血清中巨噬細胞趨化蛋白-1(MCP-1)、環(huán)氧合酶-2(COX-2)、β2-微球蛋白(β2-Mg)及尿液中β2-Mg水平;以實時熒光定量PCR(qRT-PCR)法測定大鼠腎臟尿酸轉(zhuǎn)運蛋白1(URAT1)、有機陰離子轉(zhuǎn)運體1(OAT1)、有機陰離子轉(zhuǎn)運體3(OAT3)、葡萄糖轉(zhuǎn)運體9(GLUT9)、ABC轉(zhuǎn)運蛋白G家族成員2(ABCG2)mRNA表達。結(jié)果 與模型組比較,穿山龍復方水煎液各劑量組和秋水仙堿組的腎臟系數(shù)顯著降低(P<0.01),腎損傷明顯減輕;穿山龍復方水煎液各劑量組及秋水仙堿組血清SUA、SCr、BUN、MCP-1、COX-2、β2-Mg水平及尿液β2-Mg水平顯著降低(P<0.05、0.01),UUA和UCr水平顯著升高(P<0.05、0.01);腎臟URAT1、GLUT9mRNA表達顯著降低(P<0.05、0.01),OAT1、OAT3、ABCG2 mRNA表達顯著升高(P<0.05、0.01)。結(jié)論 穿山龍復方水煎液改善GN大鼠腎損傷,作用機制與抗炎,上調(diào)OAT1、OAT3、ABCG2 mRNA水平,促進UA排泄,下調(diào)URAT1、GLUT9 mRNA水平抑制UA重吸收相關(guān)。
[Key word]
[Abstract]
Objective To investigate the protective effect of diostrodia compound decoction (DCD) on the kidney of gout arthritis (GA) & hyperuricemia (HU) -induced gout nephropathy (GN) rats. Methods Forty-eight male SD rats were divided into six groups, namely control, model, colchicine (positive controls, 0.03 mg·kg−1) and DCD high, medium and low dose (6.300, 3.150, and 1.575 mg·kg−1) groups. While moulding with sodium urine acid, potassium oxyzine, and high-fat diet, the corresponding drugs were gavaged, respectively. Kidney histopathology was observed after 7 d, Kidney index was calculated, The serum uric acid (SUA), creatinine (SCr), urea nitrogen (BUN) levels, as well as the serum levels of MCP-1, COX-2, and β2-Mg, were measured in rats, Urine uric acid (UUA), urinary creatinine (UCr) levels, as well as urine β2-Mg levels in rats, The mRNA expression of rat kidney uric acid transporter 1 (URAT1), organic anion transporter (OAT), glucose transporter, ABC transporter 9 (GLUT9), and G family members 2 (ABCG2) was determined by the Real-time PCR(qRT-PCR) assay. Results Compared with model group, the kidney index was decreased in both the dose group and the colchicine groups (P < 0.01). The levels of serum SUA, SCr, BUN, MCP-1, COX-2 and β2-Mg and urine β2-Mg in DCD groups and colchicine groups were significantly decreased (P < 0.05, 0.01), and the levels of UUA and UCr were significantly increased (P < 0.05, 0.01). The mRNA expressions of URAT1 and GLUT9 in kidney were significantly decreased (P < 0.05, 0.01), and the mRNA expressions of OAT1, OAT3 and ABCG2 were significantly increased (P < 0.05, 0.01). Conclusion DCD improved kidney injury in GN rats, and the mechanism was related to anti-inflammatory, upregulating OAT1, OAT3 and ABCG2 levels to promote UA excretion, down-regulating URAT1 and GLUT9 levels to inhibit UA reabsorption.
[中圖分類號]
R285
[基金項目]
黑龍江中醫(yī)藥大學科研基金(杰出青年培育基金)項目(2019JC02)