目的 分析姜黃連的化學(xué)成分，在物質(zhì)基礎(chǔ)層面探究姜炙對(duì)黃連的影響。方法 采用Waters Acquity UPLC BEH C18色譜柱（100 mm×2.1 mm，1.7 μm），以0.1%甲酸水溶液（A）-乙腈（B）為流動(dòng)相進(jìn)行梯度洗脫，利用LTQ-Orbitrap/MS正、負(fù)離子模式采集數(shù)據(jù)，通過(guò)高分辨質(zhì)譜數(shù)據(jù)解析，對(duì)姜黃連的化學(xué)成分進(jìn)行定性分析。結(jié)果 黃連、姜黃連和生姜汁中共鑒定出34種化合物，其中歸屬于黃連25種、姜黃連32種、生姜汁9種；黃連和姜黃連的共有成分25種，黃連姜炙后新增7種姜辣素類物質(zhì)，均為生姜汁中的成分。結(jié)論 黃連姜炙后化學(xué)成分發(fā)生變化，新增了7種姜辣素類成分，為姜黃連的藥效物質(zhì)基礎(chǔ)研究提供參考。

Objective Analyze the chemical components of Coptidis Rhizoma, and explore the effect of Coptidis Rhizoma was processed fresh ginger juice at the material basis level. Methods WatersACQUITY UPLC BEH C₁₈ column (100 mm × 2.1 mm, 1.8 μm) was used for gradient elution with 0.1% formic acid aqueous solution (A) and 0.1% acetonitrile (B) as mobile phases. The data were collected by using LTQ – Orbitrap/MS in positive and negative ion mode. The chemical constituents of Coptidis Rhizoma were analyzed qualitatively by high resolution mass spectrometry. Results Thirty – four chemical constituents were identified from Coptidis Rhizoma, Coptidis Rhizoma processed with fresh ginger juice and ginger juice, including twenty – five from Coptidis Rhizoma, Thirty–two from Coptidis Rhizoma processed with fresh ginger juice and nine from ginger juice; there are twenty-five kinds of common components in Coptidis Rhizoma and Coptidis Rhizoma processed with fresh ginger juice and seven kinds of gingerols from ginger juice were added after Coptidis Rhizoma was processed fresh ginger juice. Conclusion The chemical composition of Coptidis Rhizoma processed with fresh ginger juice was changed that seven kinds of gingerols were added. It provides a reference for the study of pharmacodynamic material basis of Coptidis Rhizoma processed with fresh ginger juice.

R284.1

國(guó)家中藥標(biāo)準(zhǔn)化項(xiàng)目：黃連等 4種中藥飲片標(biāo)準(zhǔn)化建設(shè)的炮制研究（ZYBZH-Y-HUB-20）