目的 研究黃芩莖葉總黃酮（SBTF）對結(jié)腸癌HCT116細胞凋亡、遷移和侵襲的影響。方法 采用CCK8法檢測SBTF（5、10、20、50、100、200、400、600 μg·mL^-1）對HCT116細胞增殖的影響；Annexin V/PI雙染法檢測SBTF （80、120、160 μg·mL^-1）對HCT116細胞凋亡的影響；JC-1染色法檢測SBTF對細胞線粒體膜電位的影響；Transwell小室遷移和侵襲實驗檢測SBTF對細胞遷移和侵襲的影響；Western blotting法檢測SBTF對細胞凋亡、遷移和侵襲相關(guān)蛋白表達的影響。結(jié)果 與對照組比較，SBTF可明顯抑制HCT116細胞增殖，干預(yù)24、48 h后的半數(shù)抑制濃度（IC₅₀）值分別為156.50、98.59 μg·mL^-1；SBTF顯著提高HCT116細胞凋亡率（P＜0.05、0.01）；SBTF明顯促進HCT116細胞線粒體膜電位改變；SBTF顯著降低HCT116細胞遷移和侵襲率（P＜0.05、0.01）；SBTF顯著上調(diào)HCT116細胞的Bax/Bcl-2值和cleaved Caspase-3蛋白表達（P＜0.05、0.01），顯著下調(diào)MMP-2、MMP-9蛋白表達（P＜0.05、0.01）。結(jié)論 SBTF可通過誘導(dǎo)HCT116細胞凋亡和抑制細胞遷移、侵襲發(fā)揮抗結(jié)腸癌作用。

Objective To investigate the effects of total flavonoids in Scutellaria baicalensis stem-leaf (SBTF) on the apoptosis, migration and invasion process of colon cancer HCT116 cells. Methods The effects of SBTF (5, 10, 20, 50, 100, 200, 400 and 600 μg·mL^-1) on HCT116 cells proliferation were detected by CCK8 assay. The effects of SBTF (80, 120, 160 μg·mL^-1) on cell apoptosis were detected by Annexin V/PI double staining. The effects of SBTF on mitochondrial membrane potential (MMP) were detected by JC-1 staining. The effects of SBTF on cell migration and invasion were detected by Transwell migration and invasion test. The effects of SBTF on the expression of apoptosis, migration and invasion related proteins were detected by Western blotting. Results Compared with control group, SBTF could significantly inhibit the proliferation of HCT116 cells, and the IC₅₀ values after 24 h and 48 h were 156.50 and 98.59 μg·mL^-1. SBTF significantly enhanced apoptosis of HCT116 cells (P<0.05 and 0.01). SBTF could promote the change of MMP in HCT116 cells. SBTF effectively inhibited migration and invasion of HCT116 cells (P<0.05 and 0.01). SBTF significantly up-regulated the expressions of Cleaved Caspase-3 and the ratio of Bax/Bcl-2 in HCT116 cells (P<0.05 and 0.01), and significantly down-regulated the expressions of MMP-2 and MMP-9 in HCT116 cells (P<0.05 and 0.01). Conclusion SBTF could play an anti-colon cancer role by regulating the process of apoptosis, migration and invasion of HCT116 cells.

R285.5

自貢市人工種植中藥資源現(xiàn)狀調(diào)查研究(CWKY-2018Z-03);江蘇省科技成果轉(zhuǎn)化專項項目(BA2018002)