目的 研究復(fù)方丹參浸膏中的低聚糖化學(xué)成分。方法 采用固相萃取、聚丙烯酰胺凝膠色譜、親水作用色譜（HILIC）-高效液相色譜（HPLC）-蒸發(fā)光散射檢測(cè)器（ELSD）等技術(shù)，對(duì)復(fù)方丹參浸膏中的低聚糖成分進(jìn)行分離純化，并根據(jù)理化性質(zhì)和波譜學(xué)手段進(jìn)行結(jié)構(gòu)鑒定。結(jié)果 通過(guò)方法優(yōu)化，成功分離得到了5個(gè)低聚糖成分，通過(guò)波譜分析鑒定，5個(gè)低聚糖成分分別為α-D-吡喃半乳糖-(1→6)-α-D-吡喃半乳糖-(1→6)-α-D-吡喃葡萄糖-(1→2)-α-D-呋喃果糖（1）、α-D-吡喃葡萄糖-(1→2)-β-D-呋喃果糖-(1→1)-α-D-吡喃半乳糖（2）、蔗糖（3）、果糖（4）和葡萄糖（5）。結(jié)論 化合物1、2為首次在復(fù)方丹參浸膏中分離得到。

Objective To study the chemical constituents of oligosaccharide from compound Danshen extractum. Methods The compounds were isolated and purified by SPE, HPGCP, and HILIC-HPLC. Their structures were elucidated by means of chemical evidences and spectral analyses (MS, ¹H-NMR, and ¹³C-NMR). Results Five compounds were isolated and identified as α -D-galactopyranosyl-(1-6)-α-D-galactopyranosyl-(1-6)-α-D- glucopyranosyl -(1-2)-β-D-fructofuranoside (1), α-D-glucopyranosy -(1-2)- β-D-Fructofuranose-(1-1) -α-D- galactopyranosyl (2), sucrose(3), fructose (4) and glucose (5). Conclusion Compounds 1 and 2 are isolated from compound Danshen extractum for the first time.

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