[關(guān)鍵詞]
[摘要]
目的 研究復(fù)方丹參浸膏中的低聚糖化學(xué)成分。方法 采用固相萃取、聚丙烯酰胺凝膠色譜、親水作用色譜(HILIC)-高效液相色譜(HPLC)-蒸發(fā)光散射檢測(cè)器(ELSD)等技術(shù),對(duì)復(fù)方丹參浸膏中的低聚糖成分進(jìn)行分離純化,并根據(jù)理化性質(zhì)和波譜學(xué)手段進(jìn)行結(jié)構(gòu)鑒定。結(jié)果 通過(guò)方法優(yōu)化,成功分離得到了5個(gè)低聚糖成分,通過(guò)波譜分析鑒定,5個(gè)低聚糖成分分別為α-D-吡喃半乳糖-(1→6)-α-D-吡喃半乳糖-(1→6)-α-D-吡喃葡萄糖-(1→2)-α-D-呋喃果糖(1)、α-D-吡喃葡萄糖-(1→2)-β-D-呋喃果糖-(1→1)-α-D-吡喃半乳糖(2)、蔗糖(3)、果糖(4)和葡萄糖(5)。結(jié)論 化合物1、2為首次在復(fù)方丹參浸膏中分離得到。
[Key word]
[Abstract]
Objective To study the chemical constituents of oligosaccharide from compound Danshen extractum. Methods The compounds were isolated and purified by SPE, HPGCP, and HILIC-HPLC. Their structures were elucidated by means of chemical evidences and spectral analyses (MS, 1H-NMR, and 13C-NMR). Results Five compounds were isolated and identified as α -D-galactopyranosyl-(1-6)-α-D-galactopyranosyl-(1-6)-α-D- glucopyranosyl -(1-2)-β-D-fructofuranoside (1), α-D-glucopyranosy -(1-2)- β-D-Fructofuranose-(1-1) -α-D- galactopyranosyl (2), sucrose(3), fructose (4) and glucose (5). Conclusion Compounds 1 and 2 are isolated from compound Danshen extractum for the first time.
[中圖分類(lèi)號(hào)]
R284
[基金項(xiàng)目]