目的 研究老鸛草巴布劑治療類風(fēng)濕性關(guān)節(jié)炎的藥效作用。方法 將30只雌性SD大鼠隨機(jī)分為對(duì)照組、模型組、空白制劑組、傷濕止痛膏（陽性藥）組、老鸛草巴布劑（含沒食子酸3.40 mg·g^-1）組，每組6只。除對(duì)照組外，其余各組構(gòu)建佐劑性關(guān)節(jié)炎大鼠模型：于每只大鼠右后足足趾皮內(nèi)注射弗氏完全佐劑0.1 mL致炎，對(duì)照組大鼠相同部位注射同等體積生理鹽水，第7天再次注射加強(qiáng)免疫。對(duì)照組及模型組不做干預(yù)，陽性藥組、老鸛草巴布劑組、空白制劑組分別連續(xù)21 d于大鼠右后大腿關(guān)節(jié)處貼敷傷濕止痛膏、老鸛草巴布劑、空白巴布劑。觀察大鼠體質(zhì)量及足趾腫脹度的變化；采用酶聯(lián)免疫吸附（ELISA）法測定不同組別大鼠血清中腫瘤壞死因子-α（TNF-α）、白細(xì)胞介素-6（IL-6）、血管內(nèi)皮生長因子A（VEGF-A）及白細(xì)胞介素-17（IL-17）水平；HE染色觀察各組大鼠膝關(guān)節(jié)滑膜病理變化。結(jié)果 對(duì)照組大鼠健康狀態(tài)良好，毛發(fā)順滑有光澤；模型組大鼠較對(duì)照組可見活動(dòng)、飲食減少，毛發(fā)略微發(fā)黃，足趾腫脹，關(guān)節(jié)呈急性炎癥表現(xiàn)，表面皮膚發(fā)紅發(fā)亮充血，活動(dòng)受限，但無死亡；老鸛草巴布劑組、傷濕止痛膏組大鼠狀態(tài)有所改善。HE染色結(jié)果表明，對(duì)照組大鼠關(guān)節(jié)結(jié)構(gòu)正常；模型組、空白制劑組大鼠膝關(guān)節(jié)可見滑膜細(xì)胞增生、炎性細(xì)胞浸潤并伴有血管翳生成，軟骨被侵蝕；與模型組比較，老鸛草巴布劑組與傷濕止痛膏組大鼠踝關(guān)節(jié)病變情況不同程度得到改善。與模型組比較，老鸛草巴布劑組和傷濕止痛膏組大鼠足腫脹度顯著降低（P＜0.05），血清IL-6、TNF-α、VEGF-A、IL-17水平明顯降低（P＜0.05）。結(jié)論 老鸛草巴布劑抗類風(fēng)濕性關(guān)節(jié)炎的作用良好。

Objective To study the effect of Geranium wilfordii cataplasm in treatment of rheumatoid arthritis. Methods Thirty female SD rats were randomly divided into control group, model group, blank preparation group, Shangshi Zhitong ointment (positive drug) group, and Geranium wilfordii cataplasm group (containing gallic acid 3.40 mg·g^-1) with six rats in each group. Except for the control group, the other groups were used to establish the rat model of adjuvant arthritis:0.1 mL Freund's complete adjuvant was injected intradermally into the right posterior toe of each rat to cause inflammation, and the control group was injected with the same volume of normal saline in the same part, and then injected again on the 7th day to enhance immunity. The conrol group and the model group were not intervened. The positive drug group, the Geranium wilfordii cataplasm group and the blank formulation group were respectively applied Shangshi Zhitong ointment, Geranium wilfordii cataplasm, blank poultice at the right posterior thigh joint of each rat for 21 days. The changes of body weight and toe swelling degree of rats were observed. Enzymelinked immunosorbent assay was used to determine the serum levels of tumor necrosis factor- α (TNF- α), interleukin-6 (IL-6), vascular endothelial growth factor A (VEGF-A) and interleukin-17 (IL-17) in different groups of rats. HE staining was used to observe the pathological changes of the knee joint synovium of the rats in each group. Results The rats in the control group were in good health with smooth and glossy hair. Compared with the control group, the rats in the model group showed decreased activity and diet, slightly yellowing hair, swelling of toes, acute inflammation of joints, red, shiny and congested surface skin, limited activity, but no death. The state of rats in Geranium wilfordii cataplasm group and Shangshi Zhitong ointment group was improved. The results of HE staining showed that the joint structure of rats in the control group was normal. Synovial cell proliferation, inflammatory cell infiltration, pannus formation and cartilage erosion were observed in the knee joint of rats in model group and blank preparation group. Compared with the model group, the ankle joint lesions of rats in Geranium wilfordii cataplasm group and Shangshi Zhitong ointment group were improved to different degrees. Compared with model group, the degree of foot swelling of rats in Geranium wilfordii cataplasm group and Shushizhitong ointment group was significantly decreased (P < 0.05), and the levels of serum IL-6, TNF-α, VEGF-A and IL-17 were significantly decreased (P < 0.05). Conclusion Geranium wilfordii cataplasm has good anti-rheumatoid arthritis effect.

R285.5

貴州省特色功能食品與中藥制劑開發(fā)攻關(guān)大平臺(tái)黔教合（KY字［2020］006）；貴州中醫(yī)藥大學(xué)藥用高分子材料研究中心（貴中醫(yī)黨辦發(fā)［2019］70號(hào)）；貴州中醫(yī)藥大學(xué)博士啟動(dòng)基金（貴中醫(yī)博士啟動(dòng)［2021］14號(hào)）；貴州省國內(nèi)一流學(xué)科建設(shè)項(xiàng)目（GNYL［2017］008）；貴州省藥物新劑型新工藝科技創(chuàng)新人才團(tuán)隊(duì)項(xiàng)目（黔科合平臺(tái)人才［2017］5655）