目的 探討愈風(fēng)寧心片對偏頭痛大鼠模型的鎮(zhèn)痛作用及機制。方法 SD大鼠隨機分成6組：對照組、模型組、苯甲酸利扎曲普坦片（陽性藥，0.9 mg·kg^-1）組和愈風(fēng)寧心片低、中、高劑量（378、756、1 512 mg·kg^-1）組，每天ig給藥1次，連續(xù)10 d。末次給藥后1 h，除對照組外，其余各組大鼠在頭頸處sc 10 mg·kg^-1硝酸甘油建立偏頭痛大鼠模型，觀察疼痛行為學(xué)指標，試劑盒法檢測血清中疼痛相關(guān)因子縮膽囊素（CCK）、白細胞介素-1β （IL-1β）、前列腺素E（2 PGE₂）含量；利用小鼠醋酸扭體實驗、小鼠右后足跖部福爾馬林致痛實驗觀察愈風(fēng)寧心片低、中、高劑量（546、1 092、2 184 mg·kg^-1）對小鼠扭體反應(yīng)和舔足時長的影響。結(jié)果 偏頭痛實驗結(jié)果表明，與模型組比較，愈風(fēng)寧心片各劑量組和苯甲酸利扎曲普坦片組的撓頭、甩頭次數(shù)和行為學(xué)評分顯著降低（P<0.05、0.01）；愈風(fēng)寧心片中、高劑量組血清CCK水平顯著降低（P<0.05、0.01），低、中、高劑量組血清PGE₂、IL-1β水平顯著降低（P<0.05、0.01）。醋酸扭體實驗結(jié)果表明，與模型組比較，愈風(fēng)寧心片中、高劑量組小鼠扭體次數(shù)顯著降低（P<0.05、0.01），高劑量組小鼠扭體反應(yīng)潛伏期顯著延長（P<0.01）。福爾馬林致痛實驗結(jié)果表明，與模型組比較，各給藥組均能顯著縮短小鼠的舔足時長（P<0.01）。結(jié)論 愈風(fēng)寧心片對偏頭痛模型具有鎮(zhèn)痛作用，其機制可能與調(diào)節(jié)中樞及外周神經(jīng)系統(tǒng)、改善疼痛相關(guān)因子含量有關(guān)。

Objective To investigate the analgesic effect and mechanism of Yufeng Ningxin Tablet on migraine in rats. Method SD rats were randomly divided into six groups:control group, model group, rizatriptan benzoate tablet (positive drug, 0.9 mg·kg^-1) group and Yufeng Ningxin Tablet low, medium and high dose (378, 756, 1 512 mg·kg^-1) groups. The rats were given ig once a day for 10 days. One hour after the last administration, except for control group, the rats in the other groups were treated with sc 10 mg·kg^-1 nitroglycerin in the head and neck to establish the rat model of migraine. The pain behavioral indexes were observed. The contents of pain related factors cholecystokinin (CCK), interleukin-1β (IL-1β), and prostaglandin E₂ (PGE₂) in serum were detected by kit method. The effects of low, medium and high doses of Yufeng Ningxin Tablets (546, 1 092, 2 184 mg·kg^-1) on writhing response and licking time of mice were observed by acetic acid writhing test and formalin pain induced by right hind foot in mice. Results The results of migraine experiment showed that compared with the model group, the number of head scratching, head shaking and behavioral scores of each dose group of Yufeng Ningxin tablet and rizatriptan benzoate tablet group were significantly decreased (P<0.05, 0.01). The serum levels of CCK in Yufeng Ningxin tablets medium and high-dose groups were significantly decreased (P<0.05, 0.01), and the serum levels of PGE₂ and IL-1β in Yufeng Ningxin Tablet low, medium and high-dose groups were significantly decreased (P<0.05, 0.01). The results of acetic acid writhing experiment showed that compared with model group, the writhing times of mice in Yufeng Ningxin tablet medium and high-dose group were significantly decreased (P<0.05, 0.01), and the latency of writhing reaction of mice in high-dose group was significantly prolonged (P<0.01). The results of formalin induced pain test showed that compared with the model group, each administration group could significantly inhibit the licking duration of mice (P<0.01). Conclusion Yufeng Ningxin Tablet has analgesic effect on migraine model, and its mechanism may be related to regulating central and peripheral nervous system and improving the content of pain-related factors.

R285.5

國家重點研發(fā)計劃（2019YFC1711400）