目的 探討注射用益氣復(fù)脈（凍干）（YQFM）對失血性休克大鼠的藥效作用。方法 通過股動脈放血的方法建立失血性休克大鼠模型，隨機將造模成功的大鼠分為模型組、腎上腺素（10 μg·kg^-1鹽酸腎上腺素注射液）組、YQFM低和高劑量（232.2、464.3 mg·kg^-1，464.3 mg·kg^-1為臨床等效劑量）組、聯(lián)合給藥（腎上腺素10 μg·kg^-1＋YQFM 464.3 mg·kg^-1）組，每組10只，假手術(shù)組只切口不放血。給藥結(jié)束后，觀察給藥3 h內(nèi)大鼠存活情況；使用八通道無創(chuàng)血壓儀監(jiān)測造模前、造模后、給藥3 h后的收縮壓；酶聯(lián)免疫（ELISA）法檢測各組大鼠血清中肌酸激酶同工酶（CK-MB）、乳酸脫氫酶（LDH）、丙氨酸氨基轉(zhuǎn)移酶（ALT）、天門冬氨酸氨基轉(zhuǎn)移酶（AST）和超氧化物歧化酶（SOD）水平。結(jié)果 造模后大鼠的平均動脈壓均維持在30～40 mm Hg，表明造模成功。假手術(shù)組及各給藥組大鼠在3 h內(nèi)全部存活，模型組死亡3只，存活率為70%，平均生存時間為（166.00±23.66） min。各組大鼠的基礎(chǔ)血壓無顯著性差異，模型組和各給藥組大鼠造模后收縮壓顯著降低，與假手術(shù)組比較差異顯著（P<0.001）；與模型組比較，給藥3 h后各給藥組收縮壓均顯著升高（P<0.001），聯(lián)合給藥組升壓效果最好。與模型組相比，腎上腺素組血清CK-MB、LDH、ALT、AST水平均顯著下降（P<0.01）； YQFM低劑量組LDH、ALT、AST水平顯著降低（P<0.05）； YQFM高劑量組與聯(lián)合給藥組血清CK-MB、LDH、ALT、AST水平顯著降低，SOD水平顯著升高（P<0.05、0.01、0.001），聯(lián)合給藥對血清生化指標的改善作用最好。結(jié)論 YQFM可以明顯提高失血性休克大鼠的收縮壓水平，改善血清中相關(guān)生化指標水平，且與腎上腺素聯(lián)用具有一定的協(xié)同作用。

Objective To investigate the effect of Yiqi Fumai Lyophilized Injection (YQFM) on the pharmacodynamics of hemorrhagic shock rats. Methods The hemorrhagic shock rat model was established by bleeding from the femoral artery, which were randomly divided into the model group, epinephrine group (10 μg·kg^-1), YQFM low and high dose group (232.2 and 464.3 mg·kg^-1, 464.3 mg·kg^-1 was the clinical equivalent dose), and combined administration group (epinephrine 10 μg·kg^-1 + YQFM 464.3 mg·kg^-1), 10 in each group. Rats in the sham-operation group only recieved incision without bloodletting. After administration, the survival of rats within 3 h of administration was observed. An eight-channel non-invasive blood pressure meter was used to monitor systolic blood pressure before modeling, after modeling, and 3 h after drug administration. The levels of creatine kinase isoenzyme (CKMB), lactate dehydrogenase (LDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and superoxide dismutase (SOD) in serum were detected by enzyme-linked immunosorbent assay (ELISA). Results The mean arterial pressure of the rats was maintained at 30-40 mmHg after modeling, which indicated that the models were successfully established. All the rats in the shamoperation group and the treatment groups survived within 3 h, while three rats died in the model group, with a survival rate of 70% and an average survival time of (166.00±23.66) min. There was no significant difference in the basal blood pressure of rats in each group, but the systolic blood pressure of rats in the model group and each administration group was significantly lower than that in the sham-operation group after modeling (P<0.001). Compared with the model group, the systolic blood pressure in each drug administration group was significantly increased (P<0.001), and the pressor effect in the combined administration group was the best. Compared with the model group, the levels of serum CK-MB, LDH, ALT and AST in the epinephrine group were significantly decreased (P<0.01). The levels of LDH, ALT and AST in YQFM low-dose group were significantly decreased (P<0.05). The serum levels of CK-MB, LDH, ALT and AST in YQFM high-dose group and combined administration group were significantly decreased, while SOD level was significantly increased (P<0.05, 0.01, 0.001). Combined administration had the best effect on serum biochemical indexes. Conclusion YQFM can significantly increase systolic blood pressure of hemorrhagic shock rats and improve the level of related biochemical indexes in serum. The results of our research indicated that YQFM could exert a synergistic effect with epinephrine on the hemorrhagic shock rat.

R285.5