目的 考察肉桂多次ig給予腎陽(yáng)虛大鼠后，鑒定大鼠腎臟、血液、尿液、糞便中原型成分和代謝產(chǎn)物。方法 采用ip氫化可的松方法制備大鼠腎陽(yáng)虛模型，造模成功后按照生藥量1.35g·kg^-1·d^-1ig肉桂提取物，連續(xù)15d。末次給藥后，收集大鼠血漿、尿液、糞便和腎組織樣品，進(jìn)行UPLC-LTQ-Orbitrap-MS分析。根據(jù)色譜保留時(shí)間、精確相對(duì)分子質(zhì)量、多級(jí)質(zhì)譜碎片信息結(jié)合文獻(xiàn)報(bào)道對(duì)各樣本中的成分進(jìn)行確認(rèn)，并推測(cè)代謝物的代謝途徑。結(jié)果 從腎陽(yáng)虛模型大鼠各樣本中共鑒定出24個(gè)成分，其中3個(gè)原型成分，21個(gè)代謝產(chǎn)物，包括12個(gè)原花青素類、8個(gè)木脂素類、2個(gè)二萜類、2個(gè)有機(jī)酸。結(jié)論 肉桂成分在腎陽(yáng)虛大鼠體內(nèi)代謝廣泛，代謝途徑主要包括甲基化、硫酸化和葡萄糖醛酸化反應(yīng)。尿液中檢出的化學(xué)成分最多，其次為血液、腎組織、糞便。長(zhǎng)期給藥情況下以原型存在于靶器官和血中成分很少，推測(cè)代謝物發(fā)揮藥效的可能性較大。

Objective To investigate the archetypal components and metabolites in kidney, blood, urine and feces of kidney-yang deficiency rats after repeated administration with Cinnamomum cassia.Method The rat model of kidney-yang deficiency was established by intraperitoneal injection of cortisol, and the extract of C. cassia was administered at the dosage of 1.35 g·kg^-1·d^-1 for 15 days. Plasma, urine, fecal and renal tissue samples were collected after the last administration of the extract of C. cassia and analyzed by UPLC-LTQ-Orbitrap-MS. Based on the retention time of chromatography, accurate relative molecular mass, and multilevel mass spectrometry fragment information, the components in each sample were identified and the metabolic pathways of metabolites were inferred.Results A total of 24 components (three protypes and 21 metabolites) were identified, including 12 procyanidins, 8 lignans, two diterpenoids and two organic acids.Conclusion Components of C. cassia have a wide metabolism in kidney-yang deficiency rats, and the metabolic pathways mainly include methylation, sulfation and glucuronic acid reaction. Most of the chemicals were found in urine, followed by blood, kidney tissue and feces. In the case of long-term administration, there are few components in the target organs and blood in the prototype, so it is more likely that the metabolites will exert their effects.

R285.51

國(guó)家自然科學(xué)基金面上項(xiàng)目(81673680)