[關(guān)鍵詞]
[摘要]
目的 研究商陸皂苷甲(EsA)對動脈粥樣硬化(AS)大鼠的作用及機制。方法 將SD雄性大鼠隨機分為對照組、模型組、辛伐他汀片(SIMT,10mg·kg-1,陽性對照)組和EsA高、低劑量(10、2.5mg·kg-1)組,每組6只。對照組給予普通飼料,其余各組以高脂飼料聯(lián)合ip7×105U·kg-1維生素D3(在第3天注射)制備AS模型。造模的同時ip給藥,每天1次。采用全自動生化分析儀檢測各組大鼠血清總膽固醇(TC)、三酰甘油(TG)、低密度脂蛋白膽固醇(LDL-C)和高密度脂蛋白膽固醇(HDL-C)水平;ELISA法檢測血清腫瘤壞死因子-α(TNF-α)、白細胞介素-6(IL-6)和白細胞介素-1β(IL-1β)水平;HE染色法觀察胸主動脈病理變化;Western blotting法檢測胸主動脈Toll樣受體4(TLR4)和髓樣分化因子88(MyD88)蛋白表達;免疫組化法檢測胸主動脈核因子κB(NF-κB) p65陽性細胞表達。結(jié)果 與對照組比較,模型組大鼠血清中TC、TG、LDL-C、TNF-α、IL-6和IL-1β水平顯著升高(P<0.01),HDL-C水平顯著降低(P<0.01);胸主動脈血管內(nèi)皮損傷嚴重,可見明顯藍色鈣狀斑塊;胸主動脈TLR4和MyD88蛋白表達以及NF-κB p65陽性細胞表達顯著升高(P<0.01)。與模型組比較,EsA高、低劑量組大鼠血清血脂和炎性因子水平明顯改善(P<0.05、0.01);胸主動脈血管內(nèi)皮大致恢復(fù)正常,藍色鈣狀斑塊減少;胸主動脈TLR4和MyD88蛋白表達以及NF-κB p65陽性細胞表達顯著降低(P<0.05、0.01)。結(jié)論 EsA可能通過抑制TLR4/NF-κB信號通路對大鼠AS發(fā)揮改善作用。
[Key word]
[Abstract]
Objective To investigate the effect and mechanism of esculentoside A (EsA) on rats with atherosclerosis (AS).Methods SD male rat were randomly divided into control group, model group, simvastatin (SIMT, positive drug, 10 mg·kg-1) group, EsA high and low dose (10 and 2.5 mg·kg-1) groups, with six rats in each group. The control group was given ordinary diet, and the other groups were given high fat diet combined with ip 7×105 U·kg-1 vitamin D3 (injected on the third day) to prepare AS model, while administering once a day. After rat serum collection, automatic biochemical analyzer was used to detect serum total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), and high density lipoprotein cholesterol (HDL-C) levels of rats in each group. Enzyme-linked immunoassay was used to detect serum cytokines Tumor necrosis factor-α (TNF-α), Interleukin-6 (IL-6), and Interleukin-1β (IL-1β) levels. HE staining method was used to analyze the pathological changes of thoracic aorta. Western blotting was used to detect the protein expression of Toll-like receptor 4 (TLR4) and myeloid differentiation factor 88 (MyD88). Immunohistochemical staining was used to detect the expression of nuclear factor κB (NF-κB) p65 positive cells.Results Compared with the control group, the serum levels of TC, TG, LDL-C, TNF-α, IL-6, and IL-1β in the model group were significantly increased (P <0.01), and HDL-C was significantly decreased (P <0.01). The thoracic aorta vascular endothelium of rats in the model group was severely injured, which accompanied with obvious blue calcium-like plaques. The expression of TLR4 and MyD88 proteins and NF-κB p65 positive cells in thoracic aorta were significantly increased (P <0.01). Compared with model group, the levels of serum lipids and inflammatory factors in rats in the EsA groups were significantly improved (P <0.05 and 0.01). The endothelium of the thoracic aorta returned to normal and the blue calcium plaques decreased. The expression of TLR4 and MyD88 proteins and NF-κB p65 positive cells in thoracic aorta were significantly decreased (P <0.05 and 0.01).Conclusion EsA might play a therapeutic effect on AS rats by inhibiting TLR4/NF-κB signaling pathway.
[中圖分類號]
R285.5
[基金項目]
恩施州科學(xué)技術(shù)局指導(dǎo)性項目(2018年度)