[關鍵詞]
[摘要]
目的 基于HPLC指紋圖譜、多成分定量結(jié)合化學模式識別法評價黔鉤藤質(zhì)量。方法 從貴州中藥材市場隨機購買了18批鉤藤飲片,采用HPLC法建立了18批鉤藤飲片的指紋圖譜;通過中藥色譜指紋圖譜相似度評價系統(tǒng)(2012年版)進行相似度評價,對其中5種生物堿進行了指認,并采用外標法和一測多評法進行了含量測定;采用SPSS 26.0統(tǒng)計軟件進行聚類分析和主成分分析。結(jié)果 在鉤藤飲片指紋圖譜中,確定了共有峰28個,采用對照品指認了5個生物堿成分,分別為異去氫鉤藤堿、異鉤藤堿、去氫鉤藤堿、鉤藤堿和去氫毛鉤藤堿,18批鉤藤的相似度評價表明市場上鉤藤的質(zhì)量差異較大;聚類分析將18批鉤藤飲片樣品聚為3大類;主成分分析用5個主成分進行綜合評價,其中8批鉤藤飲片的綜合得分大于1;采用一測多評法和外標法計算18批鉤藤飲片中5種生物堿的含量,經(jīng)GraphPad差異性分析表明,2種方法無顯著性差異。結(jié)論 市場上鉤藤質(zhì)量差異較大,建立了檢測5個生物堿成分的一測多評法和外標法,為完善鉤藤質(zhì)量評價提供參考。
[Key word]
[Abstract]
Objective To evaluate the quality of Uncaria rhynchophylla collected in Guizhou province based on combinative methods of HPLC fingerprint and multi-components determination and chemical pattern recognition. Methods Tottally 18 batches of U. rhynchophylla were randomly purchased from Guizhou traditional Chinese medicine market. The fingerprint of 18 batches of U. rhynchophylla was established by HPLC. The similarity of 18 batches was analyzed with Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia (2012). Five alkaloids were identified, and their contents were determined by external standard method and one test and multiple evaluations (QAMS). The hierarchical clustering analysis (HCA) and principal component analysis (PCA) were performed by SPSS 26.0. Results Totally 28 peaks were identified in the fingerprint of U. rhynchophylla and five of the common peaks were identified as isocorynoxeine, isorhynchophylline, corynoxeine, rhyncholphylline, and hirsuteine by reference substance. The fingerprint similarities of the 18 batches indicate that the quality of U. rhynchophylla in the market varied greatly. According to HCA, the samples were divided into three groups. Five principal components were selected for comprehensive evaluation by PCA, and the comprehensive scores of eight batches were greater than one. The contents of five alkaloids in 18 batches were calculated by the methods of external standard method and QAMS. GraphPad was used for difference analysis, and the results showed that there was no significant difference between the two methods. Conclusion This study showed that the quality of U. rhynchophylla differed greatly in the market, we have successfully established the multiple evaluation method and the external standard method for the determination of five alkaloid components, providing a reference for the improvement of the quality evaluation of uncaria hook.
[中圖分類號]
R284.1
[基金項目]
貴州省科技計劃項目[黔科合基礎-ZK(2021)540];貴州省科技計劃項目[黔科合基礎-ZK(2021)535];貴州中醫(yī)藥大學博士啟動基金[3043-043190010]