目的 研究跌打七厘片對小鼠骨折愈合的影響。方法 將100只C57BL/6小鼠隨機(jī)分為空白對照組20只、假手術(shù)組20只、造模組60只。造模組制備股骨骨折模型，造模后再隨機(jī)分為模型組和跌打七厘片低、高劑量（405.5、1 621.8 mg·kg^-1）組，每組20只。每天1次，連續(xù)ig給藥28 d。每周通過骨組織形態(tài)學(xué)和蘇木精伊紅（HE）染色觀察骨痂形態(tài)改變、微計算機(jī)斷層掃描分析（Micro-CT）檢測愈合骨的骨體積分?jǐn)?shù)（BV/TV），生物力學(xué)三點彎曲實驗檢測愈合骨的最大負(fù)載，全自動生化儀檢測小鼠血清鈣（Ca）、磷（P）、堿性磷酸酶（ALP）的水平。結(jié)果 HE染色、Micro-CT結(jié)果顯示，造模后第14天，與模型組比較，跌打七厘片組有較多的軟骨細(xì)胞浸潤，鈣化編織骨骨量多，新生骨痂骨量明顯較多；造模后第28天，模型組骨折斷端仍有未連接，而跌打七厘片組的骨折斷端均已連接，鈣化編織骨轉(zhuǎn)化為層狀骨，逐漸恢復(fù)骨的基本形態(tài)，有更多的新生骨痂及鈣化程度，高劑量組骨折斷端被鈣化新生骨完全填充并與周圍骨皮質(zhì)相聯(lián)合。造模后第14天，跌打七厘片高劑量組的BV/TV、最大負(fù)載顯著高于模型組（P<0.05）；造模后第28天，低、高劑量組的BV/TV、最大負(fù)載均顯著高于模型組（P<0.05）。造模后小鼠血清Ca水平各組之間沒有明顯差異。造模后小鼠血清P、ALP水平呈上升趨勢，并在21 d達(dá)到高峰，與模型組比較，第14、21天跌打七厘片低劑量組和第7、14、21、28天的高劑量組血清P水平顯著升高（P<0.05），第14、21、28天的高劑量組血清ALP水平顯著升高（P<0.05）。結(jié)論 跌打七厘片可以提高成骨活性及鈣鹽沉積，以增加骨痂水平增強骨強度，有利于小鼠骨折愈合。

Objective To study the effect of Dieda Qili Tablet on fracture healing in mice. Methods A total of 100 C57BL/6 mice were randomly divided into blank control group (n=20), sham operation group (n=20) and model group (n=60). After modeling, the model group was randomly divided into model group, Dieda Qili Tablets low and high-dose (405.5 and 1 621.8 mg·kg^-1) group, with 20 rats in each group. Once a day, continuous ig administration for 28 days. Micro-CT three-dimensional imaging analysis was used to detect the bone volume fraction of the healed bone. Biomechanical three-point bending test was used to detect the maximum load of the healed bone. Serum Ca, P, and alkaline phosphatase (ALP) contents were detected by fully automatic biochemical analyzer. Results HE staining and Micro-CT results showed that 14 days after modeling, compared with the model group, the Dieda Qili Tablets group had more chondrocyte infiltration, more calcified woven bone mass, and significantly more new callus bone mass. Totally 28 days after modeling, the fracture ends of the model group were still unconnected, while the fracture ends of the drop seven millimeter piece group were all connected. The calcified woven bone transformed into layered bone, gradually restoring the basic form of the bone, with more new callus and degree of calcification. The fracture ends of the high-dose group were completely filled with calcified new bone and combined with the surrounding bone cortex. On the 14th day after modeling, the BV/TV and maximum load of the high-dose group of Dieda Qili Tablets were significantly higher than those of the model group (P < 0.05). On the 28th day after modeling, the BV/TV and maximum load of the low and high dose groups were significantly higher than those of the model group (P < 0.05). After modeling, there was no significant difference in serum Ca levels among the groups. After modeling, the serum P and ALP levels of the model mice showed an upward trend and reached their peak on the 21st day. Compared with the model group, the serum P levels in the low-dose group of DiDa Qili Tablet on the 14th and 21st days and the high-dose group on the 7th, 14th, 21st, and 28th days were significantly increased (P < 0.05), and the serum ALP levels in the low and highdose group significantly increased on days 14, 21, and 28 (P < 0.05). Conclusion Dieda Qili Tablet has therapeutic effect on fracture in mice by improving osteogenic activity and calcium salt deposition to increase the content of callus and enhance bone strength.

R285.5

上海市中醫(yī)藥“三年行動計劃”［ZY（2021-2023）-0211］；上海市“科技創(chuàng)新行動計劃”生物醫(yī)藥科技支撐專項（20S21902100）；上海市教委協(xié)同創(chuàng)新中心：中西醫(yī)結(jié)合-中成藥臨床評價平臺（A1-U21-205-0103）；上海市申康中心示范性研究型病房建設(shè)（SHDC2022CRW010）；上海市申康中心醫(yī)企融合創(chuàng)新協(xié)同專項（SHDC2022CRT018）；上海市慢性筋骨病臨床醫(yī)學(xué)研究中心項目（20MC1920600）