目的 探討注射用益氣復(fù)脈（凍干）（YQFM）對心力衰竭感染性休克大鼠的藥效作用。方法 通過結(jié)扎冠狀動脈左前降支以及尾靜脈推注脂多糖（LPS，25 mg·kg^-1）的方法建立心力衰竭感染性休克大鼠模型，隨機(jī)將造模后大鼠分為模型組，腎上腺素（10 μg·kg^-1）組，YQFM低、高劑量（232.2、464.3 mg·kg^-1）組，聯(lián)合給藥（腎上腺素10 μg·kg^-1＋YQFM 464.3 mg·kg^-1）組，假手術(shù)組進(jìn)行同樣操作但不結(jié)扎不推注LPS。造模后即給藥，尾iv給藥1次，模型組和假手術(shù)組大鼠給予等體積的0.9%氯化鈉注射液。使用八通道無創(chuàng)血壓儀檢測造模前、造模后及給藥后大鼠收縮壓變化；ELISA法檢測各組大鼠血清中腦鈉肽（BNP）、氨基端前心鈉肽（NT-proANP）、肌酸激酶同工酶（CK-MB）、乳酸脫氫酶（LDH）、丙氨酸氨基轉(zhuǎn)移酶（ALT）、天冬氨酸氨基轉(zhuǎn)移酶（AST）和超氧化物歧化酶（SOD）水平。結(jié)果 各組大鼠的基礎(chǔ)收縮壓無顯著性差異，與假手術(shù)組比較，造模各組大鼠收縮壓顯著降低（P＜0.001）；與模型組比較，各給藥組大鼠收縮壓顯著回升（P＜0.05、0.001）。與假手術(shù)組相比，模型組血清NT-proANP、BNP、CK-MB、LDH、ALT、AST水平顯著升高（P＜0.001），SOD水平顯著降低（P＜0.001）；與模型組相比，腎上腺素組，YQFM 232.2、464.3 mg·kg^-1組和聯(lián)合給藥組的NT-proANP、BNP、CK-MB、LDH、ALT、AST水平均顯著下降（P＜0.05、0.01、0.001），腎上腺素組、YQFM 464.3 mg·kg^-1組和聯(lián)合給藥組SOD水平顯著升高（P＜0.05、0.01、0.001）。結(jié)論 YQFM可以顯著回升心力衰竭感染性休克大鼠的收縮壓水平，改善血清中相關(guān)生化指標(biāo)水平，并且與腎上腺素聯(lián)合應(yīng)用效果最好。

Objective To investigate the effect of Yiqi Fumai Lyophilized Injection (YQFM) on heart failure with septic shock in rats. Methods The animal model of heart failure with septic shock in rats were established by ligating the left anterior descending coronary artery and injecting LPS (25 mg·kg^-1) in tail vein. The rats were randomly divided into model group, epinephrine (10 μg·kg^-1) group, YQFM low dose and high dose (232.2 and 464.3 mg·kg^-1), and combined administration group (epinephrine 10 μg·kg^-1 + YQFM 464.3 mg·kg^-1). Administration immediately after molding, once in the tail iv. The rats in the model group and sham operation group were given 0.9% sodium chloride injection in equal volume. The rats of the sham operation group were performed the same operation without ligation and injection of LPS. The changes of systolic blood pressure (SBP) of rats before modeling, after modeling and after administration were detected by eight-channel non-invasive blood pressure meter. The serum levels of NT-proANP, BNP, CK-MB, LDH, ALT, AST and SOD were detected by ELISA. Results There was no significant difference in basal SBP among the groups. Except for the sham operation group, the SBP of the model rats was significantly lower than that of the sham operation group (P＜0.001). Compared with the model group, the SBP of the rats increased after administration of the corresponding drugs (P＜0.05, 0.001). Compared with the sham operation group, the levels of serum NTproANP, BNP, CK-MB, LDH, ALT and AST in the model group were significantly increased (P＜0.001), and the level of SOD was significantly decreased (P＜0.001). Compared with the model group, the levels of NT-proANP, BNP, CK-MB, LDH, ALT and AST in the epinephrine group, YQFM 232.2, 464.3 mg·kg^-1 group, and combination therapy group were significantly decreased (P＜0.05, 0.01, 0.001), and the levels of SOD were significantly increased in the epinephrine group, YQFM 464.3 mg·kg^-1 group, and combination therapy group (P＜0.05, 0.01, 0.001). Conclusion YQFM can significantly increase the SBP level of rats with heart failure and septic shock, improve the level of related biochemical indicators in serum, and have the best effect in combination with epinephrine.

R285.5