在臨床上，缺氧是引起機體損傷甚至導(dǎo)致機體死亡的最常見因素，因此建立了一系列缺氧損傷模型，如體外細(xì)胞模型上，通過液態(tài)石蠟封閉法、厭氧袋產(chǎn)氣法、混合氣體培養(yǎng)法等方法建立缺氧損傷模型。在動物模型（如大、小鼠和斑馬魚模型）上，通過混合氣體培養(yǎng)法、鈉石灰吸收法、動脈結(jié)扎法以及化學(xué)試劑處理建立缺氧損傷模型；并通過檢測缺氧后存活時間、酶學(xué)指標(biāo)、血氣指標(biāo)、組織病理切片及缺氧相關(guān)因子的變化情況進(jìn)行抗缺氧藥物的篩選和評價。綜述缺氧損傷模型及抗缺氧藥物篩選的研究進(jìn)展，以期為臨床治療缺氧提供理論依據(jù)和參考。

In clinical practice, hypoxia is the most common factor that causes organism injury or even leads to organism death. Therefore, research workers have established a series of hypoxic injury models, such as in vitro cellular models, by liquid paraffin closure method, anaerobic bag gas production method, mixed gas culture method and other methods to establish hypoxic injury models. On animal models such as rat and mouse and zebrafish models, hypoxic injury models were established by mixed gas incubation method, sodium-lime absorption method, arterial ligation method and chemical reagent treatment. And the anti-hypoxic drugs were screened and evaluated by detecting the survival time, enzymatic indexes, blood gas indexes, histopathological sections and changes of hypoxia-related factors after hypoxia. Research progress of hypoxic injury model and anti-hypoxic drug screening were summarized, in order to provide theoretical basis and reference for clinical treatment of hypoxia.

R965

泰山學(xué)者工程資助項目（tsqn202211204）；山東省優(yōu)秀青年人才基金項目（ZR2020YQ60）；濟南市“高校20條”資助項目（2021GXRC047，2020GXRC053）