目的 制備聚乙烯醇（PVA）/海藻酸鈉（SA）-聚乙烯醇（PVA）/殼聚糖（CS）雙層（PAPCS）水凝膠膜傷口敷料，并進(jìn)行質(zhì)量評(píng)價(jià)。方法 將PVA與SA以質(zhì)量比2∶1混合，配制PVA/SA混合溶液；將PVA與CS分別以質(zhì)量比1∶1、2∶1、3∶1、4∶1、5∶1混合，配制不同質(zhì)量比的PVA/CS混合溶液；采用涂布法制備PAPCS雙層水凝膠膜傷口敷料。通過水蒸氣透過率、溶脹性能、保水性、力學(xué)性能、體外凝血性能和血液相容性考察對(duì)水凝膠膜的性能進(jìn)行評(píng)價(jià)、篩選PVA與CS質(zhì)量比；通過傅里葉變換紅外光譜檢測(cè)（FTIR）和掃描電子顯微鏡（SEM）對(duì)PAPCS水凝膠膜的結(jié)構(gòu)和形貌進(jìn)行表征；通過抑菌實(shí)驗(yàn)比較PAPCS以及PAPCS復(fù)合載碘交聯(lián)環(huán)糊精金屬有機(jī)骨架（I₂@COF@PAPCS）的體外抗菌性能。結(jié)果 PVA與CS質(zhì)量比為2∶1時(shí)，PAPCS水凝膠膜綜合性能較好。PAPCS水凝膠膜為多孔結(jié)構(gòu)，具有良好的溶脹性能、保水性以及力學(xué)性能；PAPCS水凝膠膜的水蒸氣透過率為（2 643.76±91.62）g·m^-2·d^-1，接近理想范圍；與PVA/SA相比，PAPCS的凝血指數(shù)顯著降低（P＜0.01），為（72.93±3.58）%，溶血率小于5%，具有促進(jìn)血液凝固的能力且血液相容性良好；與PVA/SA相比，PAPCS對(duì)于金黃色葡萄球菌、大腸埃希菌均有明顯抑制作用，抑菌圈直徑分別為（11.89±0.22）、（12.28±0.25） mm；I₂@COF@PAPCS對(duì)金黃色葡萄球菌、大腸埃希菌的抑菌圈直徑分別為（21.95±1.47）、（18.89±0.81）mm，抑菌效果顯著優(yōu)于PAPCS（P＜0.001）。結(jié)論 采用涂布法可成功制備雙層PAPCS水凝膠膜敷料，其各項(xiàng)性能指標(biāo)良好，具有明顯的凝血、抑菌效果，與I₂@COF復(fù)合使用，抑菌作用進(jìn)一步增強(qiáng)。

Objective To prepare and evaluate the quality of polyvinyl alcohol (PVA)/sodium alginate (SA) -PVA/chitosan (CS) double-layer hydrogel film wound dressing. Methods Mix PVA and SA in a mass ratio of 2:1 to prepare a PVA/SA mixed solution. Mix PVA and CS in mass ratios of 1 : 1, 2 : 1, 3 : 1, 4 : 1, and 5 : 1 to prepare PVA/CS mixed solutions with different mass ratios. PAPCS double-layer hydrogel membrane wound dressing was prepared by coating method. The properties of hydrogel films were evaluated, and PVA to CS quality ratio was screened by water vapor permeability, swelling property, water retention, mechanical property, in vitro coagulation ability and blood compatibility. The structure and morphology of the hydrogel films were then characterized by fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Moreover, the antibacterial activity of PAPCS with I₂@COF@PAPCS in vitro was compared through inhibition zone assay. Results When the mass ratio of PVA to CS was 2 : 1, the comprehensive properties of PAPCS hydrogel membrane were better. The results suggested bilayer PAPCS hydrogel films had porous structure, good swelling performance, water retention and mechanical properties. The water vapor transmission rate of PAPCS hydrogel films was (2 643.76 ±91.62) g·m^-2·d^-1, close to the ideal range. Compared with PVA/SA, the coagulation index of PAPCS hydrogel films was significantly decreased (P < 0.01), the coagulation index was (72.93 ±3.58)%, and the hemolysis rate was less than 5%, which had good blood coagulation ability and blood compatibility. Compared with PVA/SA, PAPCS hydrogel film showed significant bacteriostasis against S. aureus and E. coli, and the diameters of the antibacterial zone were (11.89 ±0.22) and (12.28 ±0.25) mm. The diameters of antibacterial zone of I₂@COF@PAPCS against S. aureus and E. coli were (21.95 ±1.47) and (18.89 ±0.81) mm, which were significantly better than PAPCS (P < 0.001). Conclusion PAPCS hydrogel film could be prepared successfully by coating method, which had good performance indexes, obvious clotting, and bacteriostatic effects. And the bacteriostasis of PAPCS hydrogel film could be further improved by combining with I₂@COF.

R318.08

國(guó)家自然科學(xué)基金面上項(xiàng)目（82273863）