目的 建立金花茶花的高效液相（HPLC）指紋圖譜，對(duì)其化學(xué)成分進(jìn)行鑒定，并考察其體外抗氧化能力。方法 70%乙醇溶液回流法提取10批次金花茶花，采用Agilent Eclipse Plus C₁₈（250 mm×4.6 mm，5 μm）色譜柱，梯度洗脫，建立金花茶花指紋圖譜，應(yīng)用HPLC-MS法分析其化學(xué)成分，并用1，1-二苯基-2-苦基肼自由基（DPPH）和2，2-聯(lián)氮-二（3-乙基-苯并噻唑-6-磺酸）二銨鹽（ABTS）法評(píng)價(jià)金花茶花的抗氧化能力。結(jié)果 建立金花茶花共有HPLC色譜圖，標(biāo)定共有峰11個(gè)，經(jīng)過中藥色譜指紋圖譜相似度評(píng)價(jià)軟件（2012版）計(jì)算，相似度均在0.98以上。HPLC-MS分析結(jié)果顯示，金花茶花中成分多為黃酮類化合物?？寡趸芰z測結(jié)果顯示，金花茶花對(duì)DPPH自由基和ABTS自由基均具有較好的清除能力，并呈濃度相關(guān)性。結(jié)論 建立了金花茶花藥材的指紋圖譜，其含有豐富的黃酮類化合物，具有良好的體外抗氧化作用。

Objective To establish a HPLC fingerprint of Camellia nitidissima Chi, analyze its chemical components, and study its antioxidant capacity. Methods Extraction of ten batches of Camellia nitidissima Chi were made by refluxing with 70% ethanol solution. Agilent Eclipse Plus C₁₈ (250 mm×4.6 mm, 5 μm) Chromatographic column were used, gradient elution, to establish a fingerprint of Camellia nitidissima Chi, analysis of its chemical components using HPLC-MS, and evaluation of the antioxidant capacity of Camellia nitidissima Chi using DPPH and ABTS methods. Results The HPLC chromatogram of Camellia nitidissima Chi was established, and 11 peaks were calibrated. The similarity was all above 0.98 through calculation of TCM chromatographic fingerprint similarity evaluation software (2012 edition). Meanwhile, HPLC-MS analysis showed that the components of Camellia nitidissima Chi were mostly flavonoids, and the antioxidant capacity of Camellia nitidissima Chi was tested. The results showed that it had good antioxidant capacity to DPPPH free radicals and ABTS free radicals in vitro, and showed a certain concentration dependence. Conclusion We had established a fingerprint of Camea nitidissima Chi, which was rich in flavonoids and had good in vitro antioxidant effects.

R284.1

中國醫(yī)學(xué)科學(xué)院醫(yī)學(xué)與健康科技創(chuàng)新工程重大協(xié)同創(chuàng)新項(xiàng)目資助（2021-I2M-1-042）；中央高?；究蒲袠I(yè)務(wù)費(fèi)專項(xiàng)資金（3332022063）