目的 考察生姜不同時(shí)機(jī)給予正常小鼠后對(duì)其產(chǎn)熱能力的影響。方法 將C57BL/6小鼠隨機(jī)分為早上對(duì)照組、早上給姜組、晚上對(duì)照組和晚上給姜組,并分別于早、晚ig生姜水提液(以生姜生藥量計(jì)為3.25 g·kg^-1或0.9%氯化鈉溶液,連續(xù)ig 2周。測(cè)量小鼠體質(zhì)量及體溫變化,并利用冷暴露實(shí)驗(yàn)測(cè)試小鼠抗寒能力。分別于早、晚末次給姜后1 h取材,計(jì)算脂肪臟器指數(shù),HE染色觀察脂肪組織病理變化,實(shí)時(shí)熒光定量PCR(qRT-PCR)法檢測(cè)棕色脂肪和腹股溝皮下白色脂肪(iWAT)中產(chǎn)熱基因線粒體棕色脂肪解偶聯(lián)蛋白1(Ucp1)和時(shí)鐘基因(Bmal1、Per2)表達(dá)情況。結(jié)果 早上對(duì)照組和晚上對(duì)照組小鼠體質(zhì)量、體溫、抗寒能力、脂肪形態(tài)及產(chǎn)熱基因各項(xiàng)指標(biāo)均無(wú)顯著性差異。與晚上對(duì)照組相比,晚上給姜組小鼠體質(zhì)量有減輕的趨勢(shì)、給姜后體溫升高(P＜0.05)、抗寒能力顯著增強(qiáng)(P＜0.05),并顯著減小脂肪細(xì)胞形態(tài),同時(shí)顯著增加脂肪細(xì)胞中Ucp1的表達(dá)(P＜0.05),但對(duì)脂肪組織Bmal1、Per2表達(dá)無(wú)顯著影響。與早上對(duì)照組相比,早上給姜組小鼠體質(zhì)量有增加的趨勢(shì),給姜后對(duì)基礎(chǔ)體溫、抗寒能力、脂肪組織Ucp1表達(dá)無(wú)顯著影響,但顯著降低脂肪組織中Bmal1的表達(dá)、升高Per2的表達(dá)(P＜0.05)。結(jié)論 早、晚不同時(shí)機(jī)給予生姜對(duì)正常小鼠產(chǎn)熱能力具有差異化的影響,其中晚上ig生姜水提液可以顯著增強(qiáng)小鼠產(chǎn)熱能力,其機(jī)制可能與生姜影響生物鐘基因表達(dá)和促進(jìn)棕色脂肪產(chǎn)熱、白色脂肪棕色化有關(guān)。

Objective This study uses ginger as a tool drug to investigate the effects of different administration times on the thermogenesis in normal mice.Methods C57BL/6 mice were randomly divided into normal control group in the morning(MC),ginger gavage group in the morning(MG), normal control group in the evening(EC), and ginger gavage group in the evening(EG),and were given ginger water extract or normal saline in the morning or evening respectively for two weeks. The body weight and body temperature of mice were measured, and the cold exposure test was used to test the cold tolerance of mice. The mice were sacrificed at one hour after the last administration of ginger in the morning or evening, and the fat organ index was calculated. The pathological changes of adipose tissue were observed by HE staining. Real time fluorescence quantitative PCR(qRT-PCR) was used to detect the expression of mitochondrial brown fat uncoupling protein 1(Ucp1) and clock genes(Bmal1, Per2) in brown adipose tissue and subcutaneous white adipose tissue(iWAT) of the groin.Results There were no significant differences in body weight,body temperature, cold resistance, fat morphology and thermogenic genes between MC and MG group. Compared with EC group,the mice in EG group showed a trend of weight loss, a continuous increase in body temperature(P＜0.05), a significant increase in cold resistance(P＜0.05), a significant reduction in the morphology of adipocytes, and a significant increase in the expression of thermogenic gene(Ucp1) in adipocytes(P＜0.05). However, it had no effect on the expression of core clock genes(Bmal1, Per2) in adipose tissue. Compared with MC group, the MG group showed an increasing trend in body weight. It had no effect on basal body temperature, cold resistance, adipose tissue morphology and expression of thermogenic gene(Ucp1), but significantly affected the expression of Bmal1, and Per2 in adipose tissue(P＜0.05).Conclusion This study demonstrates for the first time that ginger administration at different times, in the morning or evening, has differential effects on thermogenesis in mice, and ginger administration in the evening can significantly enhance thermogenesis in mice. The mechanism may be related to the effect of ginger on the expression of circadian clock genes and the promotion of thermogenesis in brown adipose tissue and the browning of white adipose tissue.

R285.5

糖尿病認(rèn)知損傷腦單細(xì)胞轉(zhuǎn)錄圖譜及滋腎方防治配伍機(jī)制研究(22HHZYSS00015)