目的 研究紫河車凍干粉改善睡眠的作用及機(jī)制。方法 利用ip氯苯丙氨酸(PCPA)法制備大鼠失眠模型,設(shè)置對(duì)照組、模型組和紫河車凍干粉低、中、高劑量(0.09、0.18、0.36 g·kg^-1)組,每天ig給藥1次,連續(xù)給藥7 d,對(duì)照組和模型組ig等量去離子水。通過(guò)檢測(cè)體質(zhì)量增長(zhǎng)量、一般觀察、翻正實(shí)驗(yàn)、曠場(chǎng)實(shí)驗(yàn)、強(qiáng)迫游泳實(shí)驗(yàn)、懸尾實(shí)驗(yàn)評(píng)價(jià)紫河車凍干粉改善睡眠的作用;利用Western blotting技術(shù)檢測(cè)γ-氨基丁酸轉(zhuǎn)氨酶(GABA-T)、谷氨酸脫羧酶-65(GAD65)、γ-氨基丁酸轉(zhuǎn)運(yùn)體1(GAT1)蛋白的表達(dá)水平。結(jié)果 與模型組比較,紫河車凍干粉低、中、高劑量組大鼠體質(zhì)量增加量顯著升高(P＜0.01);中、高劑量紫河車凍干粉具有顯著縮短睡眠潛伏期(P＜0.05)、顯著延長(zhǎng)睡眠時(shí)間(P＜0.01)的作用;高劑量紫河車凍干粉使大鼠中央格停留時(shí)間顯著延長(zhǎng)(P＜0.01)、中央格穿格次數(shù)顯著增多(P＜0.05)、站立次數(shù)和修飾次數(shù)顯著增多(P＜0.01),說(shuō)明高劑量紫河車凍干粉能夠減輕失眠大鼠焦慮情緒,增強(qiáng)自發(fā)探索活動(dòng)能力;高劑量紫河車凍干粉使大鼠漂浮時(shí)間縮短(P＜0.05),掙扎時(shí)間延長(zhǎng)(P＜0.01),說(shuō)明高劑量紫河車凍干粉能夠減輕失眠大鼠的抑郁狀態(tài)。Western blotting結(jié)果顯示,與模型組相比,紫河車凍干粉處理組大鼠下丘腦組織中GABA-T的表達(dá)水平顯著降低(P＜0.05),GAD65、GAT-1的表達(dá)水平顯著升高(P＜0.05、0.01)。結(jié)論 紫河車凍干粉可能通過(guò)調(diào)控GABA-T、GAD65、GAT-1蛋白的表達(dá)水平,發(fā)揮改善睡眠的作用。

Objective To study the sleep-improving effect and mechanism by of Ziheche lyophilized powder(ZLP).Methods To establish the rat insomnia model by intraperitoneal injection of chlorophenylalanine(PCPA). The rats were divided into control group, model group, and low, medium, and high concentration groups(0.09, 0.18, and 0.36 g·kg^-1) of ZLP. Ig once a day for seven consecutive days, with the control group and model group receiving an equal amount of deionized water. The effect of ZLP on improving sleep was evaluated through general observation, righting experiment, open field experiment, forced swimming experiment, and tail suspension experiment, and the Western blotting technique was used to detect the expression levels of GABA-T,GAD65, and GAT-1 proteins, and preliminarily explore the mechanism of ZLP in improving sleep.Results Compared with the model group, the body weight increase of rats in the low, medium, and high dose groups of ZLP was significantly increased(P＜0.01), medium and high doses of ZLP have the effect of shortening sleep latency(P＜0.05) and prolonging sleep time(P＜0.01), a high dose of ZLP prolonged the retention time of the central grid(P＜0.01), increased the number of times the central grid was pierced(P＜0.01), and increased the number of standing and modification times in rats(P＜0.01), indicating that high-dose ZLP can reduce anxiety in insomnia rats(P＜0.05) and enhance their spontaneous exploration activity ability(P＜0.01); high dose ZLP shortened the floating time(P＜0.05) and prolonged the struggling time of rats(P＜0.01), indicating that high-dose ZLP can alleviate the depressive state of insomnia rats. Western blotting results showed that compared with the model group, the expression level of GABA-T in the hypothalamic tissue of rats treated with ZLP decreased(P＜0.05), while the expression levels of GAD65and GAT-1 increased(P＜0.05 and 0.01).Conclusion ZLP may improve sleep by regulating the expression levels of GABA-T,GAD65, and GAT-1 proteins.

R285.5

山東省科技型中小企業(yè)技術(shù)創(chuàng)新能力提升工程項(xiàng)目(2022TSGC1036,2022TSGC2003); 山東省中央引導(dǎo)地方科技發(fā)展資金項(xiàng)目(YDZX2022164)