目的 對不同產(chǎn)地金櫻子進(jìn)行綜合質(zhì)量評價,為其道地研究提供依據(jù)。方法 收集8省16批次金櫻子樣品,采用高效液相色譜法同時檢測金櫻子中薔薇酸、委陵菜酸、熊果酸、沒食子酸、兒茶素、橙皮苷、蘆丁、槲皮素、木犀草素、山柰酚、芹菜素和鞣花酸含量,建立金櫻子多組分定量控制模式;采用主成分分析(PCA)、正交偏最小二乘法-判別分析(OPLS-DA)和熵權(quán)優(yōu)劣解距離法(EW-TOPSIS)建立金櫻子質(zhì)量優(yōu)劣評價模型,對其整體質(zhì)量進(jìn)行綜合評價。結(jié)果 12個成分在各自范圍內(nèi)線性關(guān)系良好,平均加樣回收率96.94%~100.05%,RSD為0.72%~1.56%;16批樣品聚為3類;鞣花酸、兒茶素、槲皮素和沒食子酸可能是影響金櫻子產(chǎn)品質(zhì)量主要潛在標(biāo)志物;EW-TOPSIS結(jié)果顯示16批金櫻子相對貼近度(J_b)為0.146 2~0.756 2,不同產(chǎn)地的金櫻子品質(zhì)差異較大。結(jié)論 所建立的方法操作便捷,結(jié)果準(zhǔn)確,可作為金櫻子多組分定量控制方法;化學(xué)計量學(xué)及EW-TOPSIS法客觀全面,可用于金櫻子的整體質(zhì)量評價。

Objective To comprehensive quality evaluation of Rosae Laevigatae Fructus(RLF) from different producing areas, and to provide basis for studying the geoherbalism of RLF.Methods The 16 batches of RLF samples from eight provinces were collected. The high performance liquid chromatography(HPLC) method was used to simultaneous determine contents of euscaphic acid, tormentic acid, ursolic acid, gallic acid, catechin, hesperidin, rutoside, quercetin, luteolin, kaempferol, apigenin and ellagic acid, and the multi-components quantitative control mode of RLF was established. The principal component analysis(PCA) and orthogonal partial least squares-discriminant analysis(OPLS-DA) were used to screen potential markers affecting the quality of RLF.The quality evaluation model of RLF was established by using entropy weight-technique for order preference by similarity to an ideal solution(EW-TOPSIS) method, and the overall quality was evaluated comprehensively.Results The 12 components showed good linear relationships in their own ranges. The average recovery were 96.94%—100.05% with the RSDs of 0.72%—1.56%.Ellagic acid, catechin, quercetin and gallic acid, may be the main potential markers affecting the quality of RLF. The results of EWTOPSIS analysis showed that the quality evaluation closeness(J_b) of 16 batches of RLF were 0.146 2—0.756 2, The quality of RLF from different producing areas was very different.Conclusion The established HPLC method is convenient in operation and accurate in results, and can be used as a quantitative control method for multi-components of RLF. Chemometrics and EW-TOPSIS method are objective and comprehensive, and can be used for the overall quality evaluation of RLF.

R284.1

河南省高等學(xué)校重點(diǎn)科研指導(dǎo)計劃項(xiàng)目(編號20B460010)