目的 鑒定當歸-川芎藥對化學(xué)成分及大鼠ig后的入血原型成分和代謝產(chǎn)物。方法 基于超高效液相色譜-靜電場軌道阱-高分辨率質(zhì)譜技術(shù)（UPLC-Orbitrap-HRMS），結(jié)合對照品圖譜、自建數(shù)據(jù)庫、相關(guān)文獻信息及Compound Discoverer3.3、Xcalibur qual browser 4.3等軟件對當歸-川芎藥對的化學(xué)成分進行分析鑒定；大鼠經(jīng)ig給藥后制得含藥血清，通過比對含藥血清與對照組血清，鑒定入血原型成分和代謝產(chǎn)物。結(jié)果 共在當歸-川芎藥對中鑒定出69個化學(xué)成分，主要包括苯酞類24個、有機酸類24個、氨基酸類8個、含氮類7個、香豆素類4個、木脂素類1個、黃酮類1個；在含藥血清中共鑒定出30個入血原型成分，包括苯酞類15個、有機酚酸類13個、香豆素類2個；鑒定出45個代謝產(chǎn)物，包括32個苯酞類代謝產(chǎn)物，13個有機酚酸類代謝產(chǎn)物。結(jié)論 明確了當歸-川芎藥對的化學(xué)成分及入血原型成分和代謝產(chǎn)物。

Objective To identify the chemical components of Angelicae Sinensis Radix-Chuanxiong Rhizoma herb pairs and their prototype components into blood and metabolites after oral administration in rats. Methods Based on the ultra-performance liquid chromatography coupled with Orbitrap high-resolution mass spectrometry technology (UPLC-Orbitrap-HRMS), the chemical components of Angelicae Sinensis Radix-Chuanxiong Rhizoma herb pairs were analyzed and identified in combination with reference substance, self-established database, relevant literature information, Compound Discoverer 3.3, and Xcalibur qual browser 4.3 software; Drug-containing serum were prepared from normal rats by intragastric administration. The prototype components and metabolites were identified by comparing drug-containing serum with blank serum. Results A total of 69 chemical components were identified in the Angelicae Sinensis Radix-Chuanxiong Rhizoma herb pairs, including 24 phthalides, 24 organic acids, eight amino acids, seven nitrogen-containings, four coumarins, one lignan and one flavonoid. A total of 30 prototype components were identified in the drug-containing serum, including 15 phthalides, 13 organic acids, and two coumarins; 45 metabolites were identified in vivo, including 32 phthalides and 13 organic acids. Conclusion The chemical components, prototype components, and metabolites of Angelicae Sinensis Radix-Chuanxiong Rhizoma herb pairs were clarified.

R284.1

安徽省高校自然科學(xué)研究項目（KJ2021A0595）