目的 鑒定當(dāng)歸-川芎藥對(duì)化學(xué)成分及大鼠ig后的入血原型成分和代謝產(chǎn)物。方法 基于超高效液相色譜-靜電場(chǎng)軌道阱-高分辨率質(zhì)譜技術(shù)（UPLC-Orbitrap-HRMS），結(jié)合對(duì)照品圖譜、自建數(shù)據(jù)庫(kù)、相關(guān)文獻(xiàn)信息及Compound Discoverer3.3、Xcalibur qual browser 4.3等軟件對(duì)當(dāng)歸-川芎藥對(duì)的化學(xué)成分進(jìn)行分析鑒定；大鼠經(jīng)ig給藥后制得含藥血清，通過(guò)比對(duì)含藥血清與對(duì)照組血清，鑒定入血原型成分和代謝產(chǎn)物。結(jié)果 共在當(dāng)歸-川芎藥對(duì)中鑒定出69個(gè)化學(xué)成分，主要包括苯酞類(lèi)24個(gè)、有機(jī)酸類(lèi)24個(gè)、氨基酸類(lèi)8個(gè)、含氮類(lèi)7個(gè)、香豆素類(lèi)4個(gè)、木脂素類(lèi)1個(gè)、黃酮類(lèi)1個(gè)；在含藥血清中共鑒定出30個(gè)入血原型成分，包括苯酞類(lèi)15個(gè)、有機(jī)酚酸類(lèi)13個(gè)、香豆素類(lèi)2個(gè)；鑒定出45個(gè)代謝產(chǎn)物，包括32個(gè)苯酞類(lèi)代謝產(chǎn)物，13個(gè)有機(jī)酚酸類(lèi)代謝產(chǎn)物。結(jié)論 明確了當(dāng)歸-川芎藥對(duì)的化學(xué)成分及入血原型成分和代謝產(chǎn)物。

Objective To identify the chemical components of Angelicae Sinensis Radix-Chuanxiong Rhizoma herb pairs and their prototype components into blood and metabolites after oral administration in rats. Methods Based on the ultra-performance liquid chromatography coupled with Orbitrap high-resolution mass spectrometry technology (UPLC-Orbitrap-HRMS), the chemical components of Angelicae Sinensis Radix-Chuanxiong Rhizoma herb pairs were analyzed and identified in combination with reference substance, self-established database, relevant literature information, Compound Discoverer 3.3, and Xcalibur qual browser 4.3 software; Drug-containing serum were prepared from normal rats by intragastric administration. The prototype components and metabolites were identified by comparing drug-containing serum with blank serum. Results A total of 69 chemical components were identified in the Angelicae Sinensis Radix-Chuanxiong Rhizoma herb pairs, including 24 phthalides, 24 organic acids, eight amino acids, seven nitrogen-containings, four coumarins, one lignan and one flavonoid. A total of 30 prototype components were identified in the drug-containing serum, including 15 phthalides, 13 organic acids, and two coumarins; 45 metabolites were identified in vivo, including 32 phthalides and 13 organic acids. Conclusion The chemical components, prototype components, and metabolites of Angelicae Sinensis Radix-Chuanxiong Rhizoma herb pairs were clarified.

R284.1

安徽省高校自然科學(xué)研究項(xiàng)目（KJ2021A0595）