目的 建立同時測定蒺藜中槲皮素、山柰酚、異鼠李素、白楊素、蒺藜皂苷、蒺藜皂苷K、蒺藜皂苷D、蒺藜呋甾皂苷B、蒺藜苷元、?？略碥赵?、薯蕷皂苷元和替告皂苷元含量的方法，探索主成分分析（PCA）、正交偏最小二乘法-判別分析（OPLS-DA）及灰色關(guān)聯(lián)度分析（GRA）模型在蒺藜質(zhì)量差異評價中的應(yīng)用。方法 采用HPLC法，以乙腈-0.05%磷酸為流動相，梯度洗脫；檢測波長為360、203 nm；通過對多指標(biāo)成分含量結(jié)果進(jìn)行PCA、OPLS-DA分析，同時建立GRA模型，綜合分析不同產(chǎn)地蒺藜質(zhì)量差異。結(jié)果 方法學(xué)驗(yàn)證結(jié)果均符合《中國藥典》要求； 12種成分在各自范圍內(nèi)線性關(guān)系良好（r＞0.999），平均加樣回收率（n＝9）在96.92%～100.18%（RSD＜2.0%）。PCA、OPLS-DA分析顯示蒺藜苷元、異鼠李素、山柰酚和槲皮素是影響蒺藜產(chǎn)品質(zhì)量的主要潛在標(biāo)志物。GRA相對關(guān)聯(lián)度0.339 7～0.597 1，蒺藜質(zhì)量存在一定產(chǎn)地差異。結(jié)論 HPLC同時測定蒺藜中12個成分，簡便可靠，PCA、OPLS-DA和GRA可用于不同產(chǎn)地蒺藜質(zhì)量差異分析。

Objective To establish a method for the simultaneous determination of quercetin, kaempferol, isorhamnetin, chrysin, tribuloside, terrestrosin K, terrestrosin D, terrestroside B, (25R) -spirost-4-ene-3, 12-dione, hecogenin, diosgenin and tigogenin in Tribuli Fructus, and to explore the application of principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA) and grey relational analysis (GRA) in the quality difference evaluation of Tribuli Fructus. Method HPLC method was used with acetonitrile as mobile phase A and 0.05% phosphate acid solution as mobile phase B in gradient elution mode; the detection wavelengths were 360 and 203 nm. Through the chemometric analysis of the results of multi-index component content, the GRA model was established to comprehensively analysis the quality differences of Tribuli Fructus from different producing areas. Results The results of methodological verification were in line with the requirements of Chinese Pharmacopoeia; There were good linear relationships (r > 0.999) when the 12 components were in their respective ranges, and the average recovery rate (n = 9) were 96.92%—100.18% (RSD < 2.0%). Chemometric analysis showed that (25R) -spirost-4-ene-3, 12-dione, isorhamnetin, kaempferol and quercetin were the main potential markers affecting the quality of Tribuli Fructus. The relative correlation degree of GRA were 0.3397—0.5971, and there was a certain difference between producing areas of Tribuli Fructus. Conclusion The HPLC method can simultaneously determine the 12 components in Tribuli Fructus, which is simple and practical, PCA, OPLS-DA and GRA can be used to analyze the quality differences of Tribuli Fructus from different producing areas.

R284.1

甘肅省科技計劃（自籌經(jīng)費(fèi)-自然科學(xué)基金）項(xiàng)目（編號23JRRA1595）