中藥的廣泛使用,提高了肝臟毒性發(fā)生風(fēng)險(xiǎn)。斑馬魚模型在肝臟毒性研究中顯現(xiàn)出較大潛力的同時(shí),也面臨評(píng)價(jià)標(biāo)準(zhǔn)難以統(tǒng)一的問(wèn)題。在使用斑馬魚進(jìn)行藥物肝損傷檢測(cè)和評(píng)價(jià)之前應(yīng)先明確其在不同生長(zhǎng)階段的肝功能狀態(tài),利用斑馬魚胚胎和幼魚的通體透明的特點(diǎn),以胚胎的孵化率、死亡率、畸形率作出初步的發(fā)育毒性判斷,以幼魚肝臟灰度值,肝臟面積大小,卵黃囊吸收程度作為藥物是否具有肝臟毒性的判斷標(biāo)準(zhǔn),利用成魚肝臟容易分離的特點(diǎn)研究肝臟毒性的作用機(jī)制。檢測(cè)時(shí)可根據(jù)這些指標(biāo)判斷斑馬魚肝臟發(fā)育階段,為其是否發(fā)生藥物肝損傷提供依據(jù),從而進(jìn)一步提高臨床用藥安全性。綜述了不同發(fā)育時(shí)期斑馬魚評(píng)估中藥肝毒性的研究方法及研究進(jìn)展,以期為后續(xù)中藥的肝臟毒性評(píng)價(jià)提供參考。

The extensive use of Chinese medicine increases the risk of liver toxicity. While zebrafish model shows great potential in hepatotoxicity research, it also faces the problem that evaluation criteria are difficult to unify. Before using zebrafish for drug liver injury detection, the liver function status of zebrafish at different growth stages should be clearly defined. Given that the whole transparent characteristics of zebrafish embryos and juvenile fish, the preliminary developmental toxicity judgment can be made based on the hatching rate, mortality rate and deformity rate of embryos. The degree of yolk sac absorption is used as the criterion to judge whether the drug has hepatotoxicity. The mechanism of hepatotoxicity is studied by using the characteristics of easy separation of adult fish liver. These indicators can be used to determine the liver development stage of zebrafish and provide evidence for whether drug liver injury occurs, so as to further improve clinical drug safety. This paper reviewed the research methods and progress of evaluating hepatotoxicity of traditional Chinese medicine in zebrafish at different developmental stages, in order to provide reference for the subsequent detection of hepatotoxicity of traditional Chinese medicine.

R965.3;R969.4

內(nèi)蒙古自治區(qū)科技重大專項(xiàng)(2019ZD004);內(nèi)蒙古自治區(qū)科技計(jì)劃(2020GG0199)