間充質干細胞來源外泌體(MSCs-Exo)近年來成為替代間充質干細胞發(fā)揮治療作用的研究熱點,良好的外泌體分離和貯存方法是保障獲得的外泌體具有穩(wěn)定的質量和可重復的關鍵,也是臨床轉化的前提。外泌體具有多種分離方式,包括國際金標準的超速離心法,改良后的蔗糖密度梯度離心法、超濾法以及微流控技術等。貯存溫度和貯存介質是影響外泌體生物學完整性的主要因素。結合當下MSCs-Exo的研究現狀及其分離和貯存條件的研究進展進行綜述,以期為臨床轉化研究提供參考。

Mesenchymal stem cell derived exosomes (MSCs-Exo) has become a research hotpot in the field of replace Mesenchymal Stem Cell (MSCs) on the therapeutic recently. A good method of exosome separation and storage is the key to ensure the stable quality and repeatability of the obtained exosomes, and is also the premise of clinical transformation. Exosomes have a variety of separation methods, including international gold standard ultrafiltration method, improved sucrose density gradient centrifugation method, ultrafiltration method and microfluidic technology. Storage temperature and storage medium are the main factors affecting the biological integrity of exosomes. This article reviews the current research status of MSCs-Exo and their isolation and storage conditions in order to provide reference for translational research.

R965.3

中國醫(yī)學科學院醫(yī)學與健康科技創(chuàng)新工程項目(2019-I2M-5-020);天津市科技計劃項目:細胞產品開發(fā)和臨床應用的研究(22ZYJDSY00150);天津市科技計劃項目:細胞制品的成藥性及轉化研究(23ZGCXQY00050)