目的 采用生物信息學(xué)方法探究重度抑郁癥（MDD）的氧化應(yīng)激相關(guān)差異基因的表達(dá)模式及篩選相關(guān)的中藥。方法 首先通過基因表達(dá)綜合數(shù)據(jù)庫獲取數(shù)據(jù)集，利用R軟件limma包篩選差異基因，從Genecard數(shù)據(jù)庫獲取氧化應(yīng)激差異基因，利用韋恩圖映射共有基因。其次對(duì)氧化應(yīng)激差異基因進(jìn)行基因本體（GO）和京都基因與基因組百科全書（KEGG）分析，接著構(gòu)建蛋白質(zhì)-蛋白質(zhì)相互作用（PPI）網(wǎng)絡(luò)篩選氧化應(yīng)激的關(guān)鍵基因，接著進(jìn)行免疫細(xì)胞浸潤分析。最后利用Coremine Medical預(yù)測(cè)靶向中藥。結(jié)果 經(jīng)篩選共得到差異表達(dá)基因202個(gè)。KEGG富集分析集中在吞噬體，白細(xì)胞經(jīng)內(nèi)皮層遷移（TEM），脂肪細(xì)胞因子等通路。通過PPI網(wǎng)絡(luò)和LASSO確定了與MDD氧化應(yīng)激密切相關(guān)的2個(gè)關(guān)鍵基因?yàn)镸OG和AIF1。ROC曲線顯示2個(gè)關(guān)鍵基因具有很高的診斷價(jià)值。最后在GSE39653數(shù)據(jù)集中驗(yàn)證了以上Hub基因的顯著差異表達(dá)。免疫通路富集顯示，MDD組與健康組相比，靜息態(tài)的NK細(xì)胞明顯增高，T濾泡輔助細(xì)胞明顯減少。利用CoremineMedical預(yù)測(cè)人參、茶樹根、西紅花等可作為預(yù)防或治療MDD的靶向中藥。結(jié)論 通過生物信息學(xué)分析得到MDD的氧化應(yīng)激差異基因，預(yù)測(cè)了14種治療MDD的首選中藥，如蜂蜜、人參、茶樹根等。

Objective Bioinformatics methods were used to explore the expression patterns of oxidative stress-related differential genes in patients with major depressive disorder (MDD) and screen related traditional Chinese medicines. Methods Firstly, datasets were acquired through the GEO database and differential genes were screened using the limma package in R software. Differential oxidative stress genes were selected from the Genecards database, and common genes were obtained using a Venn diagram. Secondly, the differentially expressed genes related to oxidative stress were analyzed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Then a protein-protein interaction (PPI) network was constructed to identify Key genes for oxidative stress. Then the immune cell infiltration analysis was performed. Finally, Coremine Medical was used to predict targeted traditional Chinese medicines. Results After screening, a total of 202 differentially expressed genes were identified. KEGG enrichment analysis indicated that DEGs were mainly enriched in phagosome, Leukocyte transendothelial migration, Adipocytokine signaling pathway. Through the protein interaction (PPI) network and LASSO, the two key genes closely related to oxidative stress in MDD were identified as MOG and AIF1. The ROC curve showed that two Hub genes had high diagnostic value. The significant differential expression of these Hub genes was validated in the GSE39653dataset. Immunological pathway enrichment showed that compared to the healthy group, the NK cells resting in the MDD group were significantly increased, and the Follicular helper T cells were significantly reduced. Coremine Medical predicted that Ginseng Radix et Rhizoma, Camellia sinensis, Croci Stigma and other Chinese medicines could be used as targeted preventive or therapeutic measures for MDD. Conclusion The differential oxidative stress genes of MDD were obtained by bioinformatics analysis, which predicted that 14 preferred Chinese medicines for MDD were honey, ginseng and tea roots.

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國家自然科學(xué)基金面上項(xiàng)目（82301725）;大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃項(xiàng)目（20230329）;中國博士后科學(xué)基金第73批面上資助（2023M732155）;山西省科技廳基礎(chǔ)研究項(xiàng)目（202203021212028，202203021212038）;山西省高等學(xué)?？萍紕?chuàng)新項(xiàng)目（2020L0204，2022L132）;山西省衛(wèi)生健康委中醫(yī)藥科研項(xiàng)目（2023ZYYC2034）;山西醫(yī)科大學(xué)省級(jí)博士基金項(xiàng)目（SD2216）;山西醫(yī)科大學(xué)校級(jí)博士啟動(dòng)基金項(xiàng)目（XD2102）;山西醫(yī)科大學(xué)第一醫(yī)院青年項(xiàng)目（YQ2203）;經(jīng)方扶陽山西省重點(diǎn)實(shí)驗(yàn)室開放課題（CPSY202301）