目的 考察注射用丹參多酚酸（SAFI）對大腦中動脈缺血再灌注（MCAO/R）大鼠腦水腫的藥效作用。方法 線拴法構建MCAO/R大鼠模型，將造模成功的大鼠隨機分為模型組、布美他尼（陽性藥，0.2 mg·kg⁻¹）組和SAFI低、中、高劑量（5.80、11.61、23.31 mg·kg⁻¹）組，假手術組大鼠僅做血管分離，不插入線栓。每天1次、連續(xù)尾iv給藥14 d，假手術組和模型組給予0.9%氯化鈉注射液。給藥前后對各組大鼠進行稱質量和神經(jīng)功能評分；給藥后采用TTC染色法檢測腦梗死體積，稱質量法檢測腦含水量，核磁檢測腦水腫體積、水分子表觀彌散系數(shù)（ADC）；HE染色觀察腦組織病理形態(tài)。結果 給藥前大鼠體質量及神經(jīng)功能評分無差異，給藥14 d后，與模型組比較，SAFI低、中、高劑量組和布美他尼組體質量明顯增加（P＜0.01、0.001），神經(jīng)功能評分顯著降低（P＜0.01、0.001），腦組織梗死體積顯著縮小（P＜0.001），腦含水量顯著降低（P＜0.01、0.001），核磁T2加權成像（T2WI）序列顯示腦水腫體積顯著減少（P＜0.01、0.001），彌散加權成像（DWI）序列顯示ADC值顯著升高（P＜0.05、0.001）；HE染色結果顯示SAFI對神經(jīng)細胞腫大和空泡變性均有改善作用。結論 SAFI對MCAO/R大鼠腦水腫具有明顯改善作用。

Objective To investigate the effect and mechanism of Salvianolic Acids for Injection (SAFI) on cerebral edema in MCAO/R rats. Methods The rat model of middle cerebral artery ischemia-reperfusion (MCAO/R) was established by suture method. The successfully modeled rats were randomly divided into model group, SAFI low, medium, and high dose (5.80, 11.61, and 23.21 mg·kg⁻¹) group, and bumetanide (positive drug, 0.2 mg·kg⁻¹) group, with 20 rats in each group. The sham operation group and the model group were given 0.9 % sodium chloride injection once a day for 14 days. The body weight and neurological function score of rats in each group were measured before and after administration. After administration, the volume of cerebral infarction was detected by TTC staining. Brain water content was detected after administration, and nuclear magnetic detection was performed before and after administration. HE staining was used to observe the pathological morphology of brain tissue. Results There was no difference in body weight and neurological function score of rats before administration. After 14 days of administration, compared with the model group, the body weight of SAFI low middle and high dose (5.80, 11.61, and 23.21 mg·kg⁻¹) group, and bumetanide group increased significantly (P < 0.01 and 0.001), the neurological function score decreased significantly (P < 0.01 and 0.001), the infarct volume of brain tissue decreased significantly (P < 0.001), and the brain water content was significantly lower than that of the model group (P< 0.05 and 0.001). Magnetic resonance T2 weighted imaging (T2WI) sequence showed a significant decrease in brain edema volume (P < 0.01, 0.001), while diffusion-weighted imaging (DWI) sequence showed a significant increase in ADC value (P < 0.05, 0.001). HE staining results showed that SAFI could improve the swelling and vacuolar degeneration of nerve cells. Conclusion SAFI has a significant improvement effect on cerebral edema in MCAO rats.

R285.5

天津市制造業(yè)高質量發(fā)展專項資金—天津天士力之驕藥業(yè)有限公司技術中心創(chuàng)新能力建設（ZZY20232088）