目的 研究四方木皮水提物、醇提物對(duì)皮膚急性創(chuàng)傷模型大鼠的治療作用及其潛在作用機(jī)制。方法 制備四方木皮水提物、醇提物的凍干粉末，并與凡士林混合形成軟膏；將144只SPF級(jí)SD大鼠隨機(jī)分為對(duì)照組，模型組，凡士林（每只0.1 g）組，京萬紅軟膏（陽性對(duì)照，每只0.1 g）組，四方木皮水提物低、高劑量（每只0.1 g軟膏，含生藥0.05、0.10 g）組，四方木皮醇提物低、高劑量（每只0.1 g軟膏，含生藥0.05、0.10 g）組，每組各18只。除對(duì)照組外，用打孔器制成直徑為1.5 cm的2個(gè)去皮破損傷口，用0.9%氯化鈉溶液清洗傷口形成機(jī)械損傷動(dòng)物模型。造模后給藥，對(duì)照組和模型組不涂任何藥物。觀察治療后第3、7、14天大鼠的傷口愈合率；利用HE和Masson染色觀察其創(chuàng)傷組織病理變化；試劑盒法檢測(cè)創(chuàng)傷組織中羥脯氨酸含量；采用ELISA法檢測(cè)血清中白細(xì)胞介素（IL）-4、IL-6、IL-10、腫瘤壞死因子（TNF）-α、血管內(nèi)皮生長因子（VEGF）、表皮生長因子（EGF）水平；采用實(shí)時(shí)熒光定量PCR（qRT-PCR）法檢測(cè)創(chuàng)傷組織中磷脂酰肌醇激酶（PI3K）、蛋白激酶B（AKT1）、內(nèi)皮型NO合成酶（eNOS）、EGF、VEGF的基因表達(dá)水平。結(jié)果 與模型組比較，四方木皮水提物、醇提物低劑量組可顯著提高大鼠創(chuàng)面愈合率（P＜0.05、0.01），明顯降低創(chuàng)面愈合初期炎癥細(xì)胞浸潤程度，促進(jìn)創(chuàng)面處羥脯氨酸的分泌（P＜0.05、0.01），促進(jìn)膠原纖維在肉芽組織中的形成；水提物和醇取物的低劑量組均能顯著提高血清中IL-4、IL-10、VEGF、EGF水平（P＜0.05、0.01），并降低IL-6和TNF-α水平（P＜0.05、0.01），同時(shí)在創(chuàng)傷部位提高PI3K、AKT1、VEGF和EGF的基因水平（P＜0.05、0.01），降低eNOS基因水平（P＜0.01）。結(jié)論 四方木皮提取物對(duì)急性創(chuàng)傷有一定的治療作用，其作用機(jī)制可能與激活EGFR-PI3K/Akt通路相關(guān)。

Objective To study the therapeutic effect of Saracae Cortex extract on acute skin trauma model rats and its potential mechanism. Methods The freeze-dried powder of aqueous and alcohol extracts of Saracae Cortex was prepared and mixed with petrolatum to form ointments. A total of 144 SPF-grade SD rats were randomly divided into the control group, model group, petrolatum group (0.1 g per rat), Jingwanhong ointment group (positive control, 0.1 g per rat), low and high-dose aqueous extract of Saracae Cortex groups (0.1 g per rat, containing 0.05 and 0.10 mg of crude medicine, respectively), and low and high-dose alcohol extract of Saracae Cortex groups (0.1 g per rat, containing 0.05 and 0.10 mg of crude medicine, respectively), with 18 rats in each group. Except for the control group, two 1.5 cm diameter full-thickness skin wounds were created using a puncher, and the wounds were washed with 0.9% sodium chloride solution to establish a mechanical injury animal model. After modeling, the drugs were administered. The control group and the model group were not treated with any drugs. The wound healing rates of rats on the 3rd, 7th, and 14th days after treatment were observed. HE and Masson staining were used to observe the pathological changes of the wound tissues. The content of hydroxyproline in the wound tissues was detected by a kit. The levels of cytokines such as interleukin (IL)-4, IL-6, IL^-10, tumor necrosis factor (TNF)-α, vascular endothelial growth factor (VEGF), and epidermal growth factor (EGF) in the serum were detected by ELISA. The gene expression levels of phosphatidylinositol 3-kinase (PI3K), protein kinase B (AKT1), endothelial nitric oxide synthase (eNOS), EGF, and VEGF in the wound tissues were detected by real-time fluorescence quantitative PCR (qRT-PCR). Results Compared with the model group, the low-dose groups of aqueous and alcohol extracts of Saracae Cortex significantly increased the wound healing rate of rats (P < 0.05, 0.01), significantly reduced the degree of inflammatory cell infiltration in the early stage of wound healing, and promoted the secretion of hydroxyproline at the wound site (P < 0.05, 0.01), and promoted the formation of collagen fibers in granulation tissue. The low-dose groups of both aqueous and alcohol extracts significantly increased the levels of IL-4, IL-10, VEGF, and EGF in the serum (P < 0.05, 0.01), and decreased the levels of IL-6 and TNF-α (P < 0.05, 0.01). At the same time, they increased the gene levels of PI3K, AKT1, VEGF, and EGF at the wound site (P < 0.05, 0.01), and decreased the gene level of eNOS (P < 0.01). Conclusion Saracae Cortex extract has a certain therapeutic effect on acute trauma, and its mechanism may be through the activation of EGFR-PI3K/Akt pathway.

R285.5

廣西壯族自治區(qū)大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃項(xiàng)目（ S202210600057，S202310600104）;廣西壯族自治區(qū)中醫(yī)藥管理局中醫(yī)藥民族醫(yī)藥自籌科研項(xiàng)目（ gzzc1127）