目的 研究刺五加注射液對化療藥物阿霉素誘導(dǎo)心肌損傷的保護(hù)及助眠、抗抑郁作用，為其臨床的輔助治療應(yīng)用提供實(shí)驗(yàn)依據(jù)。方法 1）大鼠ip 2.5 mg/kg的鹽酸阿霉素，每周1次，連續(xù)6周，誘導(dǎo)大鼠心臟毒性模型；于阿霉素注射后立即尾靜脈輸注藥液，時(shí)長1 h，共6次，對照組、模型組給予生理鹽水，受試藥組給予刺五加注射液（50、100、200 mg/kg，以總黃酮計(jì)），陽性對照組給予參芪扶正注射液（25 mL/kg）；測定大鼠血清心肌酶、炎性因子，以及心肌組織病理改變。2）以地西泮（0.5 mg/kg）為陽性對照，測定刺五加注射液（30、60、120 mg/kg）對戊巴比妥鈉閾劑量（45 mg/kg）及閾下劑量（30 mg/kg）致小鼠睡眠的影響。3）采用小鼠懸尾實(shí)驗(yàn)，以氟西?。?0 mg/kg）為陽性對照，測定刺五加注射液（30、60、120 mg/kg）對不動(dòng)時(shí)間的影響，觀察其抗抑郁作用。結(jié)果 與模型組比較，刺五加注射液50、100、200 mg/kg不同程度減輕心肌病理改變，減少心肌酶乳酸脫氫酶（LDH）、肌酸激酶同工酶MB（CK-MB）漏出，除刺五加注射液50 mg/kg組CK-MB外，均差異顯著（P<0.05）；可使IL-1β、IL-6、TNF-α水平不同程度降低，其中100、200 mg/kg組均差異顯著（P<0.05、0.01）。與對照組比較，刺五加注射液30、60、120 mg/kg劑量相關(guān)性地顯著縮短小鼠睡眠潛伏期（P<0.05、0.01、0.001），120 mg/kg組顯著延長睡眠時(shí)間（P<0.001），60、120 mg/kg組顯著增加入睡率（P<0.05、0.01）。與對照組比較，刺五加注射液各劑量組明顯縮短小鼠懸尾不動(dòng)時(shí)間（P<0.05、0.01）。結(jié)論 刺五加注射液對化療藥物阿霉素導(dǎo)致的心肌損傷治療效果明顯，而且能助眠、抗抑郁，這些作用對于恢復(fù)機(jī)體體力、促進(jìn)健康有重要作用。

Objective To investigate the protective effect of Acanthopanax Injection on cardiotoxity induced by doxorubicin in rats, and the hypnotic effect together with antidepressant effect of Acanthopanax Injection was researched in mice. Methods 1) The cardiotoxicity model of rats was induced by ip injection of doxorubicin hydrochloride with 2.5 mg/kg once a week for 6 Weeks. The total cumulative dose was 15 mg/kg. The serum myocardial enzymetissue were determined after Acanthopanax Injection treatment. 2) The sleep-promoting effects of Acanthopanax Injection on the threshold dose (45 mg/kg) and subthreshold dose (30 mg/kg) of sodium pentobarbital were studded in mice. 3) The time of immobility is recorded in the tail suspension test (TST) in mice. The normal group and model group were given NS, and the Acanthopanax Injection groups were given 50, 100, 200 mg/kg in the rats, and 30, 60, 120 mg/kg in the mice respectively; the positive control was given Shenqi Fuzheng Injection 25 mL/kg in rats, diazepam 0.5 mg/kg, and fluoxetine 20 mg/kg in mice,respectively. Results After treatment with 50, 100, and 200 mg/kg of Acanthopanax Injection in rats,myocardial pathological changes were reduced to different degrees, with less leakage of myocardial enzyme LDH and CK-MB. As the mice treated by Acanthopanax Injection at the dose of 30, 60, 120 mg/kg, the sleep incubation period shortened,the sleep time prolonged, and the sleeping rate increased. The time of immobility was significantly shortened in TST with Acanthopanax Injection administration. Conclusions Acanthopanax Injection has a protective effect of on cardiotoxity induced by doxorubicin in rats, Acanthopanax Injection also shows hypnotic and antidepressant effects in mice, which are important for restoring the body's physical strength and promoting health.