目的 研究鉤藤人參合用對高血壓合并心衰（HCHF）模型大鼠的影響。方法 將大鼠隨機(jī)分為假手術(shù)組、模型組、卡托普利組（0.014 3 g/kg）和鉤藤人參合用高（鉤藤0.9 g/kg+人參1.8 g/kg）、中（鉤藤0.45 g/kg+人參0.9 g/kg），低（鉤藤0.225 g/kg+人參0.45 g/kg）劑量組。采用腹主動脈縮窄術(shù)制作大鼠HCHF模型，5周后通過大鼠尾動脈壓監(jiān)測儀評估造模是否成功，其中假手術(shù)組只分離腹主動脈，不結(jié)扎。酶聯(lián)免疫吸附法（ELISA）測定大鼠血清B型腦鈉肽（BNP）、血管緊張素Ⅱ （Ang Ⅱ）、血管緊張素1型受體（AT₁）、β1腎上腺素受體（β1-AR）水平。取心臟測定心臟質(zhì)量指數(shù)（HWI），HE染色法觀察大鼠心肌纖維排列狀況。結(jié)果 造模第5周后手術(shù)各組收縮壓較對照組顯著升高（P<0.01），驗證模型成功；給藥第2、4周，鉤藤人參合用中、高劑量組收縮壓較模型組顯著下降（P<0.05、0.01）。與模型組比較，鉤藤人參合用中、高劑量組能顯著降低心衰大鼠的HWI以及AT₁、Ang Ⅱ、BNP的濃度，顯著升高β1-AR濃度（P<0.05、0.01），且呈劑量相關(guān)性。光鏡下觀察模型組心肌結(jié)構(gòu)紊亂，心肌細(xì)胞排列呈波浪狀，心肌纖維斷裂嚴(yán)重；卡托普利組和人參鉤藤高劑量組可見心肌結(jié)構(gòu)排列較均勻，心肌細(xì)胞形態(tài)正常，心肌纖維略有斷裂。結(jié)論 鉤藤人參合用對于降低大鼠血壓，改善大鼠心衰癥狀效果顯著，且呈劑量相關(guān)性，其中高劑量與卡托普利組效果相當(dāng)。

Objective To study the effects of combination of Uncaria rhynchophylla and Panax ginseng on hypertension complicated with heart failure (HCHF) in rats. Method Rats were randomly divided into sham-operated group, model group, captopril group(0.0143 g/kg), high (Uncaria 0.9 g/kg + ginseng 1.8 g/kg), middle (Uncaria 0.45 g/kg + ginseng 0.9 g/kg), and low (Uncaria 0.225 g/kg + ginseng 0.45 g/kg) dose combination of Uncaria and ginseng groups. Rats with HCHF model was produced by abdominal aortic coarctation. After 5 weeks, assessing the success of the model through rat tail artery pressure monitoring. In the sham operation group, only abdominal aortas of rats were isolated without ligation. Enzyme-linked immunosorbent assay (ELISA) was used for determination of rat serum BNP, AngⅡ, AT₁, and β1-AR. Cardiac quality index (HWI) was measured by heart test, and myocardial fiber arrangement was observed by HE staining. Results Systolic blood pressure (SBP) was significantly higher in each group than that in the control group (P<0.01) after the 5th week of model establishment, and the model was verified to be successful. At the 2nd and 4th week of administration, the SBP of the medium and high dose groups of combination of Uncaria and ginseng was significantly lower than that of the model group (P<0.05, 0.01). Compared with the model group, each dose group of combination can significantly reduce the HWI and the concentration of AT₁, AngⅡ, and BNP in rats with heart failure (P<0.05), and significantly increaseβ1-AR concentration (P<0.05) in a dose-dependent manner. Under light microscope, we found that the myocardial structure of the model group was disordered, the myocardial cells arranged in a wavy manner, and the myocardial fiber was severely broken. In the captopril group and the high-dosecombination group, the myocardial structure was arranged more evenly, the myocardial cell morphology was normal, and the myocardial fiber was slightly broken. Conclusion Combination of Uncaria and ginseng has a significant effect on lowering blood pressure and improving symptoms of heart failure in rats. Among them, the high-dose combination group has the best effect, and has the same effect as the captopril group.

國家自然科學(xué)基金青年基金資助項目（81102549）