目的 進(jìn)行魚腥草注射液與新魚腥草素鈉注射液體外人肝微粒體代謝研究，為進(jìn)一步闡明中藥多成分體系體內(nèi)代謝規(guī)律奠定基礎(chǔ)。方法 制備人肝微粒體，用Brad ford法測定酶活性，配制肝微粒體體外溫孵體系，加入魚腥草注射液與新魚腥草素鈉注射液溫孵，用氣相色譜-質(zhì)譜聯(lián)用（GC/MS）法測定并比較魚腥草注射液及新魚腥草素鈉注射液代謝樣品圖譜。結(jié)果 檢測到魚腥草注射液的人肝微粒代謝產(chǎn)物20個，新魚腥草素鈉注射液肝微粒代謝產(chǎn)物24個，魚腥草注射液多成分體系與新魚腥草素鈉注射液單成分經(jīng)肝微粒體代謝其產(chǎn)物的數(shù)目不完全相同，但產(chǎn)物結(jié)構(gòu)類型一致，均為脂肪族、芳香族、萜類揮發(fā)性成分，其中兩者的代謝產(chǎn)物中均有己醛、α-哌烯、桉樹腦、癸酸、庚醇、2-異丙基-5-甲基-1-庚烷6種成分，分別占代謝產(chǎn)物總量的15.40%和49.14%。結(jié)論 多成分代謝與單成分代謝規(guī)律有所不同，但均呈多成分群聚集的特點(diǎn)，成分之間存在與原植物相似的代謝途徑，符合中藥各成分在同一網(wǎng)絡(luò)體系相互連通、交流、傳遞的“網(wǎng)通性”規(guī)律。

Objective Studying the metabolism of Houttuyniae and sodium new Houttuyfonate injection in liver microsomes to lay a foundation for clarifying the metabolism rule of Chinese herbal multi components system in vivo and Determining the Q-Marker of houttuynia. Methods The liver microsome was prepared and the enzymatic activity was determined by Brad ford method. The in vivo iver microsomes incubating system was prepared, and the Houttuyniae injection and sodium new Houttuyfonate injection were incubated in the liver microsomes; the metabolic product were determined by GC-MS. Results Houttuynia injections incubated by liver microsomes had 20 metabolites, and 24 metabolites of sodium new Houttuynia injections, of which there were 6 common products. The number of products metabolized by hepatic microsomes in the multi-component system of sodium new Houttuyfonate injection was not exactly the same as that in the single component of Houttuynia cordata injection, but the structure types of products were the same, they were all aliphatic, aromatic and terpene volatile components. The same six metabolites of both components are hexanal, alpha-piperine, eucalyptus, decanoic acid, heptanol alcohol and 2-isopropyl -5-methyl -1-heptane, accounting for 15.40% and 49.14% of the total metabolites respectively. Conclusion The regulation of multicomponent metabolism is different from that of single component, but both of them have the characteristics of multicomponent group aggregation. There is a metabolic pathway similar to the original plant among components. It is in accordance with the law of "network communication" that the components of Chinese medicine are connected, exchanged and transmitted in the same network system.

天然藥物活性物質(zhì)與功能國家重點(diǎn)實驗室開放課題資助（GTZK201607）；湖南中醫(yī)藥大學(xué)化學(xué)工程與技術(shù)一流學(xué)科建設(shè)項目資助，湖南省教育廳優(yōu)秀青年基金項目（2015B172）；湖南省中醫(yī)藥科研基金（201494）；湖南省教育廳一般項目（2015C1039）；湖南省“十二五”省級藥學(xué)重點(diǎn)學(xué)科（1007）開放基金項目資助