目的 通過(guò)對(duì)金芪降糖片血清藥物化學(xué)研究方法的建立，探討其口服吸收后的入血成分。方法 利用超高效液相色譜-電噴霧串聯(lián)四級(jí)桿質(zhì)譜（UPLC-ESI-MS）和多重反應(yīng)監(jiān)測(cè)（MRM）對(duì)金芪降糖片樣品及ig給予金芪降糖片后大鼠血清樣品進(jìn)行分析，比較各樣品分析結(jié)果，確認(rèn)金芪降糖片的血中移行成分。結(jié)果 ig給予金芪降糖片后大鼠血清中發(fā)現(xiàn)16種原形產(chǎn)物，分別為新綠原酸、綠原酸、隱綠原酸、咖啡酸、木蘭花堿、蘆丁、金絲桃苷、木犀草苷、異綠原酸A、異綠原酸C、黃連堿、表小檗堿、藥根堿、小檗堿、巴馬汀、黃芪甲苷。結(jié)論 確定了金芪降糖片的血中移行成分，為闡明其有效成分及作用機(jī)制提供參考。

Objective To explore the absorbed constituents in plasma after oral administration of Jinqi Jiangtang Tablets determined by its serum pharmacochemistry research method. Methods Using UPLC-ESI-MS and multiple response monitoring (MRM) method, the chromatograms of rat serum samples after oral administration of Jinqi Jiangtang Tablets were analyzed and compared with samples of rats in the control group, in order to identify the constituents absorbed into blood of Jinqi Jiangtang Tablets. Results A total of 16 original form compounds absorbed into the blood after ig administration of Jinqi Jiangtang Tablet were detected, including Neochlorogenic acid, Chlorogenic acid, Cryptochlorogenic acid, Caffeic acid, Magnoflorine, Rutin, Hyperoside, Luteoloside, Isochlorogenic acid A, Isochlorogenic acid C, Coptisine, Epiberberine, Jatrorrhizine, Berberine Palmatine, and AstragalosideA. Conclusion The constituents absorbed into blood after oral administration of Jinqi Jiangtang Tablets were identified in this study, which is conductive to the exploration of bioactive constituents of Jinqi Jiangtang Tablets and its mechanism of action.

國(guó)家自然科學(xué)基金項(xiàng)目（21502142）；天津市應(yīng)用基礎(chǔ)與前沿技術(shù)研究計(jì)劃（15JCQNJC14100）