目的 建立橘葉UPLC指紋圖譜，對不同產(chǎn)地藥材進(jìn)行質(zhì)量評價，為科學(xué)評價各批次橘葉質(zhì)量提供依據(jù)。方法 采用Acquty UPLC BEH C₁₈（100 mm×2.1 mm，1.7 μm）色譜柱，以0.2%甲酸水溶液-甲醇為流動相進(jìn)行梯度洗脫，體積流量為0.3 mL/min，柱溫為26℃，檢測波長為330 nm。結(jié)合聚類分析和主成分分析對20批不同產(chǎn)地橘葉藥材的整體質(zhì)量進(jìn)行評價。結(jié)果 建立20批橘葉的UPLC對照指紋圖譜，標(biāo)定27個共有峰，聚類分析分成4類，利用主成分分析進(jìn)行驗證且確定6個決定峰。結(jié)論 結(jié)合化學(xué)計量學(xué)和UPLC指紋圖譜為建立全面有效的橘葉藥材質(zhì)量控制模式提供參考。

Objective To establish fingerprint of Folium Citri Reticulatae by UPLC, and assess the quality of different habitats, provide reasonable reference for its quality evaluation and control. Methods The ACQUTY UPLC BEH C₁₈ (100 mm×2.1 mm, 1.7 μm) was used with a mobile phase of methanol-0.2% pheosphoric acid in gradient elution. The flow rate was 0.3 mL/min. The column temperature was 26℃, and detection wavelength was 330 nm. Principal component analysis and cluster analysis were used to evaluate 20 batches quality of Folium Citri Reticulatae from different habitats. Results Fingerprints of 20 batches of samples were established and 27 common peaks were obtained. All of samples could be classified into four cluste by cluster analysis, and PCA was used to verify and identify 6 determination peaks. Conclusion The combination of Chemometrics (CMM) and fingerprint can provide a reference for the comprehensive and effective quality control of herbs.

國家重大科技專項重大新藥創(chuàng)制——中醫(yī)藥優(yōu)勢領(lǐng)域的創(chuàng)新中藥關(guān)鍵技術(shù)開發(fā)研究（2017ZX09301005）