目的 建立高效液相色譜法（HPLC）同時測定附子山茱萸聯(lián)合給藥后大鼠心肌組織中三磷酸腺苷（ATP）、二磷酸腺苷（ADP）、一磷酸腺苷（AMP）、磷酸肌酸（PCr）和肌酐5種物質(zhì)含量的方法，用于慢性心衰大鼠心肌組織樣品能量物質(zhì)的測定。方法 考察色譜條件（色譜柱、流動相、柱溫、檢測波長、進樣量、洗脫梯度和洗脫時間等）；評價該方法的精密度、線性和回收率、檢測限與定量限、穩(wěn)定性等。結(jié)果 專屬性、線性、精密度、回收率、穩(wěn)定性均良好，能滿足后續(xù)研究的需要。動物給藥后能量物質(zhì)均有不同程度升高，且聯(lián)合給藥效果更顯著。結(jié)論 方法檢測結(jié)果可靠、操作簡便；檢測方法可用于慢性心衰大鼠心肌組織樣品能量物質(zhì)的測定。

Objective Establish a method for the simultaneous determination of five substances (ATP, ADP, AMP, PCr and creatinine) in myocardium of rats by high performance liquid chromatography (HPLC). Methods The chromatographic conditions (column, mobile phase, column temperature, detection wavelength, injection volume, elution gradient and elution time) were investigated. The method was evaluated for precision, linearity and recovery, limit of detection and limit of quantization, stability, and so on.Results Specificity, linearity, precision, recovery and stability were all good, which could meet the need of follow-up study. After administration, the energy and substance of animals all have different degrees of increase.Conclusion The method is reliable and easy to operate. The detection method can be used for the determination of energy substances in myocardial tissue of chronic heart failure rats.

廣東省自然科學(xué)基金（N0.2016A030313646）；廣東省科技計劃項目（廣東省實驗動物與動物實驗技術(shù)綜合創(chuàng)新平臺建設(shè)（一期），2017A070702001）